AFB1致肝癌作用短期体内实验模型的长期观察及绿茶和BHA对AFB1诱发大鼠肝癌的影响

 200只大鼠分为4组,各组均先施以AFB₁致肝癌作用短期体内实验模型的基本程序。在此基础上,3个实验组在AFB₁期间分别饲用0.5%BHA、0.2%BHA及5%绿茶饲料,对照组此时用正常饲料。短期模型结束后,再进行长期观察。结果发现,0.5%BHA组的r-GT灶最少、最小,肝癌发生率最低、对照组的r—GT灶最多、最大,肝癌发生率最高。这提示:（1）本短期模型对于AFB₁诱发肝癌方面的基础研究是适用的。（2）r-GT灶的数量及大小与肝癌发生率有平行关系,故其在本模型中是可靠的肝癌前病变的标志。(3)绿茶及BHA均有抑制AFB₁致肝癌的作用。

A LONC-TERM OBSERVATION OF THE SHORT-TERM AFBI INDUCED HEPATOCARCINOGENESIS TEST MODEL AND THE EFFECTS OF GREEN TEA AND BHA ON THE LONG-TERM TEST

Two handred male wistar rats were divided into three exper imental groups (A～C) andpone control group (D). All groupsreceived the same basic program of the short-term AFBI-indueeb hepatoeareinogenesis test model, i. e., during the eriod of AFB_1 ip., group A, B and C were fed with0.5%BHA, 0.2% BHA ands% green tea diet respectively while the group D was fed with normal diet only. Rats in each group were sacrificed at the end of the short-term model (sth week) or at the end of the long-term odservation (92th week). Results showed not only r-GT foci were most numerous and largest but also the incidence of PLC was highest in control group, while these values were lower in green tea and BHA group. The results suggest: (1) This short-term test model is fairly suitable for investigating AFB_1-induced hepatocarcinogenesis. (2) The number and size of r-GT foci were in good corelation with the incidence of PLC, so they are reliable in this short-term model. (3) Both green tea and BHA have inhibitory effect on AFBl-induced hepatocarinogenesis.