神经干细胞移植治疗脊髓损伤

背景:研究已证实神经干细胞能促进脊髓损伤大鼠神经功能的恢复,但对移植细胞在体内的增殖、分化、迁移的研究有限。目的:观察神经干细胞移植对脊髓损伤大鼠后肢运动功能修复的影响。方法:SD大鼠制成T10脊髓全横断损伤模型,于造模成功后1周采用局部微量注射法。随机数字表法分为3组:损伤对照组仅打开椎管暴露脊髓;移植对照组:注射10μLDMEM/F12培养液;细胞移植组:造模后移植浓度为1.0×109L-1的神经干细胞悬液10μL。移植后通过不同时间点BBB行为评分、病理组织学、免疫荧光技术评价大鼠脊髓功能修复情况及移植细胞在体内的存活、迁移、分化。结果与结论:在体外成功建立SD大鼠海马源性神经干细胞培养体系;移植对照组、细胞移植组大鼠随着时间延长BBB评分均不同程度提高,从移植后2周起细胞移植组大鼠评分明显高于移植对照组(P<0.05);神经干细胞移植后能够在体内继续存活、迁移并且分化为NF-200、GFAP表达阳性的神经元及星形胶质细胞。提示神经干细胞移植治疗脊髓损伤是一种有效的方法。

Neural stem cells transplantation for the treatment of spinal cord injury

BACKGROUND:Many studies showed that neural stem cells(NSC) transplantation can promote functional improvements in spinal cord injury(SCI) rats.However,the activity of proliferation,differentiation and migration of the transplanted NSCs in the injured zone remain poorly understood.OBJECTIVE:To investigate the effects of NSCs transplantation on the function recovery of rat hind limb with SCI.METHODS:SD rats were randomized into 3 groups after the animal models of spinal cord completely transsected at T10 had been established for 1 week.Control injuried group only exposed spinal cord;10 μL DMEM/F12 fluid and 10 μL NSCs(1.0×109/L) were transplanted into the caulal zone of the spinal cord in the transplantation control and NSCs groups,respectively.The function repair was evaluated by Bundle branch block(BBB) score and pathology;Imunnoflurore-scence technique was used to detect the surviveal,migration and differentiation of the Brdu-labeled cells in vivo.RESULTS AND CONCLUSION:A cultivation of NSCs derived from rat hampocampus on proliferation and differentiation in vitro was established successfully;The BBB scores of the transplantation control and NSCs groups were increased with time prolonged,which was more greater in the NSCs group from 2 weeks after transplantation(P < 0.05);NSCs could continue survive and migrate into the injured parts in vivo after transplantation,and some of them could differentiated into NF-200 and the glial fibriuary acidic protein positive cells that had characteristics of neuron or astroglia.It suggested that NSCs transplantation is an effective method to repair SCI in rats.