A rapid method for the inactivation of virus infectivity prior to assay for interferons |
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| Authors: | A D Barrett N Hunt N J Dimmock |
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| Institution: | 1. Department of Biological Sciences, University of Warwick, Coventry CV4 7AL U.K.;2. Heinrich P Institut für Experimentelle Virologie and Immunologie, Universität Hamburg, Martinistrasse 52, D-20 Hamburg 20, F.R.G. |
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| Abstract: | Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of alpha, beta or gamma mouse interferons. |
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| Keywords: | beta-propiolactone inactivation of virus assay of interferons α β γ |
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