1,2-二氯乙烷对大鼠肝细胞内游离钙离子浓度的影响

目的了解1，2-二氯乙烷染毒24h对大鼠肝细胞的损伤及其机理。方法运用显微荧光术测定了大鼠肝细胞内游离钙离子浓度，同时，测定了大鼠肝细胞培养上清液乳酸脱氢酶（LDH）活力作为大鼠肝细胞受损的指标。结果所有剂量组的1，2-二氯乙烷的大鼠肝细胞内游离钙离子浓度与对照组比较差异均无显著性（P〉0．05）；LDH活力仅在浓度为5mmol／L的1，2-二氯乙烷染毒组与对照组比较差异也无显著性（P〉0．05）；而1，2-二氯乙烷其他染毒组（即浓度为10mmol／L和20mmol／L）与对照组比较差异均有显著性（P〈0．01）。结论较高浓度（10mmol／L和20mmol／L）的1，2-二氯乙烷能损伤大鼠肝细胞，损伤的途径不是通过破坏肝细胞内钙稳态机制。

Effect of 1,2-Dichloroethane on Cytoplasmic Free Ca2+ Concentration in Rat Hepatocytes

Objective To explore the damaging mechanism of action in rat hepatocytes exposed to 1,2-dichloroethane (DCE). Methods The cytoplasmic free Ca~(2+) concentration in rat hepatocytes were measured by microfluorometry. Meanwhile, the damage of rat hepatocytes were evaluated with measurement of lactate dehydrogenase (LDH) activity in the supernatant of the culture medium and determination of cytoplasmic free Ca~(2+) concentration. Results There was no sifnificant difference between cytoplasmic free Ca~(2+) concentrations of rat hepatocytes exposed to DCE and that of control group (P>0.05), but the LDH activity in the supernatant of culture medium in the groups exposed to 10 mmol/L and 20 mmol/L DCE was significantly higher than that of control group (P<0.01). Conclusion The mechanism of damaging action to rat hepatocytes was not due to disturbance of cytoplasmic free Ca~(2+) concentration in rat hepatocytes.