外阴阴道念珠菌病小鼠模型阴道组织趋化因子MCP-1和MIP-2 mRNA水平测定

目的 探讨趋化因子在白念珠菌性阴道炎模型小鼠阴道组织中的表达情况.方法 采用雌激素化小鼠念珠菌性阴道炎模型,另以雌激素化但未接种白念珠菌组、接种等量白念珠菌但未雌激素化组为对照组,动态观察小鼠阴道灌洗液的真菌生长情况,并在接种后不同时间随机摘取小鼠的阴道组织,半定量RT-PCR方法检测组织内单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎性蛋白-2(MIP-2)mRNA的表达情况.结果 与未雌激素化的小鼠比较,雌激素化并接种白念珠菌小鼠阴道灌洗液自第2天开始即可发现念珠菌生长并持续到接种后21d,自第4天起阴道组织内有高水平的MCP-1 mRNA表达,并持续高于另外两组直到观察期末.3组小鼠的MIP-2 mRNA表达水平在接种后第2天明显高于完全阴性对照组,但以后各时间点3组间两两比较差异无统计学意义.结论 MCP-1 mRNA高水平表达可能与白念珠菌持续的阴道感染过程有关,而MIP-2在感染过程中的作用不明显.

Expression of MCP-1 and MIP-2 mRNA in Vagina of Murine Model of Vulvovaginal Candidiasis

Objective To investigate the kinetic expression level of chemokines (MCP-1 and MIP-2) in vagina of a murine model of vulvovaginal candidiasis (VVC).Methods The estrogen-treated murine model of VVC was set up.Vaginal specimens were obtained in different duration after inoculation of C.albicans intravaginally.Semi-quantitative RT-PCR was applied to determine MCP-1 and MIP-2 mRNA levels in these tissues.Results Compared with the control mice treated with olive oil,persistent growth of C.albicans was found from the 2nd day to 21st day after inoculation in estrogen-treated mice.Significantly higher levels of MCP-1 mRNA were observed in vaginal tissues in infected estrogen-treated mice than those in other 2 groups,infected but non estrogen-treated mice and estrogen-treated but uninfected mice.The high level of MCP-1 mRNA maintained from the 4th day to 21st day in infected estrogen-treated mice.It was also found that levels of MIP-2 mRNA were significantly higher in the vagina in the 2nd day in 3 groups of mice than those in naive mice,however,no significant difference was shown among 3 groups throughout the study period.Conclusion High level of MCP-1,rather than MIP-2,may be associated with susceptibility to VVC.