INTESTINAL BLOOD FLOW IN THE DOG MEASURED BY INDICATOR FRACTIONATION WITHOUT TISSUE SAMPLES

A modification of Sapirstein's indicator fractionation technique for measurement of intestinal blood flow has been studied and evaluated in the dog. The concentration of ⁴²K within the intestinal wall was measured simultaneously by a tissue assay procedure and by use of a miniature Geiger-Müller tube within the intestinal lumen. Both methods gave similar results under a wide variety of conditions. Isotope measurement by the Geiger-Müller tube technique obviates the need for tissue samples to be taken and would permit repeat measurements of intestinal blood flow in the same animal.