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| 反相高效液相色谱分析赤霉素GA3含量 | |
| 引用本文： | 朱曙东,赵琛,赵文君,赵升皓. 反相高效液相色谱分析赤霉素GA3含量[J]. 药学学报, 1993, 28(10): 788-791 |
| 作者姓名： | 朱曙东 赵琛 赵文君 赵升皓 |
| 作者单位： | 徐州医学院生物化学研究室，徐州医学院生物化学研究室，徐州医学院生物化学研究室，徐州医学院生物化学研究室 徐州 221002，徐州 221002，徐州 221002，徐州 221002 |
| 摘 要： | 以μBondapak C18柱建立了赤霉素GA3的HPLC分析方法。反相HPLC梯度条件可使GA3与包括双键异构体GA1在内的其它同系物通过离子配对机制获得分离。此法操作简便,不需衍生化;分析快速,色谱全程仅15 min;检测灵敏,0.5μg即可准确定量;线性(r=0.999,n=5)、重复性(RSD<2%,n=4)均佳;结果准确。优越性全面超出各传统分析方法,可望成为赤霉素质量分析的标准方法。 |
| 关 键 词： | 赤霉素A2(GA3) 反相高效液相色谱 |
| 收稿时间： | 1993-02-25 |
DETERMINATION OF GIBBERELLIN A3 BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| SD Zhu,S Zhao,WJ Zhao and SH Zhao. DETERMINATION OF GIBBERELLIN A3 BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J]. Acta pharmaceutica Sinica, 1993, 28(10): 788-791 | |
| Authors: | SD Zhu S Zhao WJ Zhao SH Zhao |
| Abstract: | The analysis of gibberellin GA3 by high performance liquid chromatography (HPLC) was carried out with μBondapak C18 column. The developed reversed phase HPLC procedure enables the separation of different GAs with high resolution by means of ionic suppression, including the separation of double bond isomers GA1 and GA3. This method is convenient (without the necessity of derivatization), rapid (15 min for one overall run), sensitive (0. 5 μg is sufficient for precise assay), and has fine linearity (r= 0.999, n=5) and reproducibility (relative standard deviation<2%, n=4). |
| Keywords: | Gibberellin A_3 (GA_3) Reversed phase high performance liquid chromatography |
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