商陆多糖I对小鼠脾淋巴细胞增殖及脾淋巴细胞、巨噬细胞分泌细胞因子的影响

商陆多糖Ⅰ(Phytolacca acinosa polysaccharides Ⅰ,PAP-Ⅰ)体外能显著促进小鼠脾淋巴细胞增殖,促进丝裂原诱导的淋巴细胞转化及双向混合淋巴细胞反应。检测PAP-Ⅰ和小鼠脾淋巴细胞培养上清液发现PAP-Ⅰ可显著促进小鼠脾淋巴细胞产生白细胞介素2(IL-2)及集落刺激因子(colony stimulating factor,CSF)。PAP-Ⅰ和小鼠腹腔巨噬细胞培养上清液中也存在CSF活性及促进重组粒单系集落刺激因子诱导骨髓细胞增殖的细胞因子。PAP-Ⅰ,ip可显著促进小鼠脾淋巴细胞增殖及IL-2产生。

EFFECTS OF PHYTOLACCA ACINOSA POLYSACCHARIDE ON SPLENIC LYMPHOCYTE PROLIFERATION AND CYTOKINE SECRETION FROM SPLENIC LYMPHOCYTE AND MACROPHAGE

The effects of Phytolacca acinosa polysaccharides Ⅰ (PAP-Ⅰ), a polysaecharide extracted from Phytolacca acinosa Roxb: on splenic lymphocyte proliferation and cytokines production from splenic lymphocyte and macrophage were studied. Lipopolysaceharides (LPS) and PAP-Ⅰ were found to significantly augment splenic lymphocyte proliferation of normal BALB/c, nude BALB/e and NC mice in vitro, but concanavalin A (Con A) was shown to stimulate only normal BALB/c and nude BALB/c splenic lymphocyte proliferation. Also, PAP-Ⅰ significantly enhanced Con A or LPS-indueed lymphocyte proliferation and mixed lymphocyte reaction. Significant enhancement of colony stimulating factor (CSF) production was observed from splenic lymphocyte of normal BALB/c and nude BALB/c mice but not from NC mice when treated with PAP-Ⅰ for 5 d. PAP-Ⅰ was shown to significantly enhance interleukin-2 (IL-2) production from normal mice splenocyte and Con A stimulated normal mice splenocyte in a concentration-dependent fashion. Supernatant of PAP-treated macrophage (MΦ) were collected and CSF activity was tested. The results confirmed that PAP-Ⅰ can significantly stimulate MΦ to secret CSF activity on d 1. The supernatant also contained a cytokine which exhibited a synergistic action with recombinant murine granular—macrophage CSF (RMGMCSF) to stimulate mice bone marrow cell proliferation. PAP-Ⅰ, 5～50 mg · kg^-1, ip can enhanced splenic lymphocyte proliferation and IL-2 production. These findings indicate that PAP-I can augment immunologic function in vitro and in vivo.