阿托伐他汀对缺氧再复氧脐静脉内皮细胞CD40配体表达的影响

目的研究不同浓度阿托伐他汀对缺氧/再复氧诱导的人脐静脉内皮细胞CD40配体表达水平的影响,探讨阿托伐他汀对内皮细胞损伤的保护机制。方法采用培养的人脐静脉内皮细胞,给予缺氧1 h,再复氧3 h处理,建立缺氧/再复氧损伤模型,给予不同浓度阿托伐他汀预处理,通过流式细胞仪测定脐静脉内皮细胞CD40配体表达水平的变化。结果缺氧/再复氧处理后脐静脉内皮细胞CD40配体表达水平显著增高,阿托伐他汀可下调缺氧/再复氧所诱导的细胞CD40配体的表达,并随其药物浓度的增高(0.1、1、5、10μmol/L),CD40配体的表达逐渐下降。结论他汀类药物可以下调内皮细胞CD40配体的表达,并随其浓度的增加下调作用更明显,从而发挥其调脂外的对缺氧/再复氧内皮保护和抗炎等作用。

Effect of atorvastatin on CD40 ligand expression in HUVECs induced by hypoxia/reoxygenation injury

Objective To investigate the effect of atorvastatin on CD40 ligand expression in human umbilical vein endothelial cells （HUVECs） induced by hypoxia/reoxygenation （H/R） injury. Methods HUVECs were cultured with atorvastatin （0. 1, 1, 5, 10 μnol/L）, and stimulated with H/R injury. CD40 ligand expression levels in HUVECs were detected by flow cytometry （FCM） analysis. Results In HUVECs, exposure to H/R injury resulted in a marked increase in CD40 ligand expression; Atorvastatin attenuated the expression of CD40 ligand exposed to H/R injury in a dose- dependent manner, with a maximal effect at concentration of 5 μmol/L. Conclusions Atorvastatin can inhibit CD40 ligand expression induced by H/R injury. These results may have important implications for the understanding of the mechanism of anti-inflammatory and immunological effect of statins.