| 高效液相色谱法测定葛根总黄酮及制剂中葛根素的含量 |
| |
| 引用本文： | 郭建平,孙其荣,周全. 高效液相色谱法测定葛根总黄酮及制剂中葛根素的含量[J]. 药学实践杂志, 1996, 0(5): 300-301,299 |
| |
| 作者姓名： | 郭建平 孙其荣 周全 |
| |
| 作者单位： | 解放军第２７２医院，第二军医大学药学院 |
| |
| 摘 要： | 以反相高效液相色谱法（ＲＰ－ＲＰＬＣ）测定葛根总黄酮提取物及制剂中葛根素的含量。检测波长２５０ｎｍ，流动相为甲醇－水（２５：７５），进样２０μｌ采用外标法。当葛根素浓度在０．５－１２μｇ／ｍｌ时，浓度与峰面积呈良好线性关系，ｒ＝０．９９９１。回收率及ＲＳＤ分别为１００．９％，１．２８％。
|
| 关 键 词： | 反相高效液相色谱法；葛根；葛根素；愈风宁心片；葛根总黄酮；葛根黄酮控释胶囊 |
RP-HPLC method for the determination of puerarin in isoflowonesd from Radix puerariae and its preparations |
| |
| Guo Jianping,Shun Qirong and Zhou Quan. RP-HPLC method for the determination of puerarin in isoflowonesd from Radix puerariae and its preparations[J]. The Journal of Pharmaceutical Practice, 1996, 0(5): 300-301,299 |
| |
| Authors: | Guo Jianping Shun Qirong Zhou Quan |
| |
| Affiliation: | No.272 PLA's Hospital Tianjin 300020 |
| |
| Abstract: | A RP-HPLC method for the determination of Puerarin in isoflowonesd from Radix Puerariae and its preparations and a C 18 column and a mobile phase of methanol-water (25:75) were employed.Puerarin was determined at 250nm.The recovery rate of the Puerarin was 100.9%.The rigression equation was X=466.83 8501.25C(r=0.9991),The method is simple,sensitive and accurate. |
| |
| Keywords: | RP-HPLC puerarin |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《药学实践杂志》浏览原始摘要信息 |
|
点击此处可从《药学实践杂志》下载全文 |
