Effect of arginase on splenic killer cell activity in patients with gastric cancer

Arginase has been detected in high levels in gastric cancer tissues. The effect of arginase on the activities of splenic natural killer (NK) cell, phytohemagglutinin activated killer (PAK) cell, and interleukin-2 activated killer (LAK) cell in patients with gastric cancer (N=12) was evaluatedin vitro. These activities in patients (N=10) with trauma and benign lesions were used as control. The splenic NK and PAK cell activities in patients with gastric cancer were significantly lower than in the controls (P<0.05), whereas LAK cell activity did not have significant difference. Arginase inhibited all splenic killer cell activities to a similar degree between patients with gastric cancer and the controls. The inhibition was dose-related. These data suggest that arginase may play a positive role in the spread of gastric cancer cells. However, LAK may be a potential approach of immunoadoptive therapy in the future.This study was supported by a grant from National Science Council of the Republic of China (NSC 77-0412-B075-61).