A novel mutation occurring in the PHO80 gene suppresses the PHO4 c mutations of Saccharomyces cerevisiae |
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| Authors: | Hideki Okada Akio Toh-e |
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| Affiliation: | (1) Department of Fermentation Technology, Hiroshima University, Shitami, Saijo, 724 Higashihiroshima, Japan;(2) Laboratory of Genetics, Department of Biology, University of Tokyo, Hongo, 113 Tokyo, Japan |
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| Abstract: | Summary We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isoleucine for methionine 42 of the Pho80 protein. Substitution of valine (PHO80-3) or leucine (PHO80-4) for the same methionine by site-directed mutagenesis also suppressed PHOc. Suppression by PHO80-2) did show some allele specificity. From these results we were able to delimit the region of PHo80 which may interact with the Pho4 protein. |
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| Keywords: | PHO Saccharomyces Protein-protein interaction Regulation |
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