HPLC同时测定双青咽喉片中3种活性成分的含量

 目的建立HPLC同时测定双青咽喉片中枸橼酸、没食子酸和绿原酸含量的方法。方法色谱柱:Dikma Kromasil C₁₈柱(4.6mm×250mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;分段变波长测定;柱温:30℃;流速:0.8mL·min^-1。结果枸橼酸、没食子酸和绿原酸的线性范围分别为:0.6024～6.024μg(r=0.9999),0.0312～0.312μg(r=0.9999),0.2016～2.016μg(r=0.9999),平均回收率分别为98.56%,99.13%,98.26%,RSD为0.95%,0.78%,1.37%。结论该方法简便、快速、灵敏、准确、具有良好的重复性和回收率,可作为该制剂的质量控制标准。

Simultaneous Determination of Three Active Constituents in Shuangqing Yanhou Tablets by HPLC

OBJECTIVE To establish a HPLC method for the simultaneous determination of citric acid,gallic acid and chlorogenic acid in Shuangqing Yanhou tablets.METHODS The separation was carried out on a Dikma Kromasil C₁₈ column(4.6 mm×250 mm,5 μm) with gradient elution.The mobile phase was acetonitrile-0.1% phosphoric acid solution.The samples were measured at multiple wavelengths.The column temperature was 30 ℃.The flow rate was 0.8 mL·min^-1.RESULTS The calibration curves were linear in the ranges of 0.602 4-6.024 μg(r=0.999 9) for citric acid,0.031 2-0.312 μg(r=0.9999)for gallic acid,0.201 6-2.016 μg(r=0.999 9)for chlorogenic acid.The average recoveries were 98.56% with RSD 0.95% for citric acid,99.13% with RSD 0.78% for gallic acid,98.26% with RSD 1.37% for chlorogenic acid.CONCLUSION This method is simple,rapid,sensitive,accurate and with good repeatability and recovery,it can be used as a quantitative analysis for this preparation.