| 二硫化碳对大鼠支持细胞凋亡及FasL表达的影响 |
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| 引用本文： | 邓菁,陈国元,季佳佳,谭皓,王松,虎凤仙,柴莲花,刘四海,刘卫东.二硫化碳对大鼠支持细胞凋亡及FasL表达的影响[J].中华劳动卫生职业病杂志,2006,24(12):730-733. |
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| 作者姓名： | 邓菁 陈国元 季佳佳 谭皓 王松 虎凤仙 柴莲花 刘四海 刘卫东 |
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| 作者单位： | 1. 430030,武汉,华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,环境与健康教育部重点实验室
2. 430030,武汉,华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,预防医学实验教学中心 |
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| 基金项目： | 国家自然科学基金资助项目（30471442） |
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| 摘 要： | 目的探讨二硫化碳对离体培养支持细胞凋亡及FasL基因表达的影响。方法以0、0．36、0．72、1．44μmol/ml浓度的二硫化碳作用于离体培养的大鼠支持细胞24 h后,应用噻唑蓝(MTT)法检测支持细胞存活率,流式细胞术检测细胞凋亡及逆转录聚合酶链反应(RT-PCR)技术检测凋亡相关基因FasL的表达。结果二硫化碳处理后的支持细胞存活率随作用浓度的增加而下降,当≥1．44μmol/ml浓度时,细胞存活率(73．34%±1．39%)明显下降,与溶剂对照组(99．98%±5．48%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度升高,细胞凋亡率增加,当浓度≥1．44μmol/ml时,支持细胞凋亡率(7．93%±0．43%)明显升高,与溶剂对照组(3．63%±0．53%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度增加,凋亡相关基因FasL表达量亦明显增加。结论二硫化碳对离体培养支持细胞具有细胞毒性作用,可导致细胞凋亡率升高,凋亡相关基因FasL高表达。
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| 关 键 词： | 二硫化碳 细胞凋亡 基因 |
| 收稿时间： | 2005-11-28 |
| 修稿时间： | 2005年11月28 |
Apoptosis and gene FasL expression induced by carbon disulfide in rat steroli cells |
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| DENG Jing,CHEN Guo-yuan,JI Jia-jia,TAN Hao,WANG Song,HU Feng-xian,CHAI Lian-hua,LIU Si-hai,LIU Wei-dong.Apoptosis and gene FasL expression induced by carbon disulfide in rat steroli cells[J].Chinese Journal of Industrial Hygiene and Occupational Diseases,2006,24(12):730-733. |
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| Authors: | DENG Jing CHEN Guo-yuan JI Jia-jia TAN Hao WANG Song HU Feng-xian CHAI Lian-hua LIU Si-hai LIU Wei-dong |
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| Institution: | Department of Labor Health & Environment Health, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China. |
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| Abstract: | OBJECTIVE: To study apoptosis and gene FasL expression induced by carbon disulfide in steroli cells of male rats. METHODS: Steroli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively. RESULTS: Steroli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05). CONCLUSION: CS(2) is cytotoxic to steroli cells. It could cause apoptosis of steroli cells. |
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| Keywords: | Carbon disulfide Apoptosis Genes |
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