新疆哈萨克族食管癌p16抑癌基因缺失及其表达的研究

目的：探讨抑癌基因在哈萨克族（哈族）食管癌发生、发展中的作用，应用分子生物学技术，阐明哈族食管癌高发的机理。方法；应用ＰＣＲ瑜民泳法和ＬＳＡＢ免疫组织化学分析法对新疆地区原发性食管癌及其正常组织中Ｐ１６基因第３外显子纯合缺及及基因表达进行检测。结果：４１对标本中癌组织及其正常组均未发现纯合缺失，２８例标本中１２例有Ｐ１６基因表达，占４２．９％（１２／２８），其中哈族为５３．８％（７／１３），汉族为

Study on the homozygous deletion and expression of the tumor suppressor gene p16 in Xinjiang Kazak's esophageal cancer

Objective: To explore mutation of tumor suppressor gene p16 in Kazak's primary esophageal cancer,and their relationship in the carcino-genesis and progression. Methods: We used PCR combined with PAGE and immunohistochemistry to detect homozygous deletion of p16 exon 3 and expression of pl6 in patients with primary esophageal cancer. Results: No homozygous deletion of p16 exon 3. Expression of pl6 gene in 12 out of 28( 42.9 % ) ES samples were detected, among them in 7 out of 13(53.8 % )Kazak's and in 5 out of 15(33.3 % )Han's esophageal cancer were detected respectively. Conclusion: These results suggest that homozygous deletion of p16 gene exon 3 may not be involved in the pathogenesis and progression of esophageal cancer but there is close relationship between the expression of p16 and tumor grading and clinical staging.