枳椇子提取物对实验大鼠肝组织中TIMP-1与MMP-13表达的影响

目的：探讨枳子提取物对实验大鼠肝组织基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。方法：SD大鼠48只,随机分为2组：正常对照组（16只）与模型组（32只）,用四氯化碳制备大鼠肝纤维化模型,共造模6周,6周末各组分别处死大鼠8只,取肝脏进行HE染色；模型组大鼠随机分为3组：自然恢复组、对照组和给药组,于12周末处死各组大鼠,取肝脏,运用半定量逆转录聚合酶链反应（RT-PCR）检测肝组织中MMP-13 mRNA与TIMP-1 mRNA的表达。结果：MMP-13 mRNA的表达在各组之间并无显著性差异,而TIMP-1 mRNA在各组之间有显著性差异,与正常组相比,自然恢复组大鼠肝组织TIMP-1 mRNA显著升高(P＜0.05),而与自然恢复组相比,对照组和给药组大鼠肝组织TIMP-1 mRNA的表达显著降低（P＜0.05),其中给药组TIMP-1 mRNA比对照组显著降低（P＜0.05)。结论：枳子提取物逆转肝纤维化的机制可能是减少肝纤维化大鼠肝脏中TIMP-1 mRNA的表达,逐渐恢复肝脏胶原降解系统,从而逆转肝纤维化。

Effect of Hovenia dulcis extract on expression of MMP-13 and TIMP-1 in hepatic tissue

Objective: To investigate the effects of Hovenia dulcis extract on mRNA expression of MMP-13 and TIMP-1 mRNA in hepatic tissue in experimental rats.Method: 48 male Sprague-Dawley rats were randomly divided into 2 groups:normal group(16) and model group(32),hepatic fibrosis was induced by CCl_4 for 6 weeks in rats,8 rats were sacrificed at the end of the 6~(th) week from every group respectively,HE staining of hepatic tissue was performed;In the model group,rats randomly subdivided into 3 groups:spontaneous recovery group,control group and medication administration group,8 rats were sacrificed at the end of the 12~(th) week from every group respectively,the mRNA levels of MMP-13 and TIMP-1 in hepatic tissue were assayed by semi-quantitative RT-PCR.Result: The mRNA expression of MMP-13 among the 4 groups were not statistically significant,but the mRNA expression of TIMP-1 among the 4 groups were statiscally significant.The levels of TIMP-1 mRNA were significantly increased in control group and medication administration group compared,with those in the model group((P<0.05)),and reverse effects of medication administration groups were significantly high than those of control group(P<0.05).Conclusion: Inhibition of the mRNA expression of TIMP-1 may be the mechanism of reversing hepatic fibrosis H.dulcis,for thus collogen degradation system was recoveried gradually.