HPV的快速检测与分型

以通用引物PCR初筛158例标本，凡HPV感染阳性标本再用型持异引物PCR作分型检测，结果：疣、乳头瘤等良性病变的HPV检出率为61．1％（55／90），主要检出HPV6和／或HPV11，占阳性例数的89．1％（49／55）；食管癌组织有较高的HPV感染率（66．2％，45／68），以检出HPV6，11，16为主，并明显高于HPV8（X2检验，P＜0．01），提示HPV感染可能与本地区食管癌发生密切用关。本研究建立的HPV快速检测和分型方法，用于临床检测和回顾性研究均可获得满意结果。

Rapid detection and typing of human papillomaviruses

Human papillomavirus (HPV) DNA in 158 specimens were detected using general primers - Polymerase Chain Reaction (GP- PCR). The positive specimens were typed using type - specific primers Polymerase Chain Reaction (TSP- PCR). The total positive rate of HPV- DNA was 63.3% (100/158). The positive rate of HPV in warts and papillomas was 61.1% (55/90) and The major infection types of HPV were HPV - 6 and/or HPV- 11. It also showed that tha types of HPV were closely related to papillomas and warts in Shantou region. There was a higher detectable rate (45 of 68, 66.2%) of HPV DNA in esophageal carcinoma. The HPV DNA types detected were mainly HPV- 6/11 and HPV16. Our result suggested that HPV infection might be closely associated with the pathogenesis of the esophageal carcinoma in Shnatou region.Our method detection and typing of HPV - DNA is recommendable to investigating clinic samples.