转化生长因子－β1诱导前单核自血病细胞分化过程中细胞表型变化的研究

本文对重组人转化生长因子－β１诱导前单核白血病细胞系ＴＨＰ－１细胞向ＭΦ分化过程中细胞表面特异性抗原表达变化进行了研究。流式细胞仪分析表明，实验细胞ＴＨＰ－１主要表现单核细胞的表型，巨噬细胞标志Ｍａｃ－１α、Ｍａｃ－１β、Ｍａｃ－２为阴性。ｒｈＴＧＦ－β１作用后，分化细胞表面单核细胞标志４Ｆ２表达下降，巨噬细胞特异抗原Ｍａｏ－１α、Ｍａｃ－１β、Ｍａｃ－２表达上升，细胞表现出ＭΦ表型。结合前文分化细胞表现出的脱氢酶硝基蓝四唑还原、α－萘酚醋酸酯酶活性和吞噬功能，可以发现分化细胞在细胞表面特异性抗原表达及细胞表型的转变与其ＭΦ生物学功能的获得在时相上基本一致。

The Alterations of Specific Cell Surface Markers During the inducedDifferentiation of Human Promonocytic Leukemia(THP-1)Cells byrhTGF-β1

The alterations of specific cell surface differentiation antigens during the induced differentiation of THP-1 cells by rhTGF-β1 was studied by FACS analysis witha series of Mo/MΦhmonoclonal antibodies.It appeared that THP-1 cells reacted mainly with 4F2 antibodies against specificmonocyte surface antigen, and the specific markers for macrophages were almostnegative.Upon induction with rhTGF-β1,THP-1 cells differentiated into macrophage-like cells which gradually,exhibited with positive surface markers of Mao-1α,Mac-1β,and Mac-2 in up to 80-90% of the cells and reduced greatly their monocytic antigen expression.These results were in concordance with the previous findings in thefunctional and cytochemical analysis of differentiated MΦ-like cells.