葡萄籽提取物对小鼠急性化学性肝损伤保护作用的实验研究

目的：研究葡萄籽提取物（GSE）对四氯化碳（CCl4）所致小鼠急性化学性肝损伤的保护作用，并探讨其可能的作用机制，为进一步研究GSE的保健功能奠定基础。方法：雄性昆明种小鼠60只，随机分为正常对照组、急性化学性肝损伤模型组、联苯双酯阳性对照组（150mg／kg）以及GSE低、中、高剂量组（50、250、500mg／kg）共6组。测定并比较各组小鼠肝脏和脾脏系数，采用赖氏法测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性，采用黄嘌呤氧化酶法测定肝组织中超氧化物歧化酶（SOD）活性，采用硫代巴比妥酸法（TBA）测定肝组织中丙二醛（MDA）含量，并对各组进行比较，观察肝脏病理组织学变化。结果：GSE各剂量组均能升高急性化学性肝损伤小鼠血清ALT和AST活性（P〈O．01），升高肝组织SOD活性（P〈O．01），降低MDA含量（P〈O．01），并能不同程度地改善肝脏病理组织损伤。结论：葡萄籽提取物对CCl4所致急性化学性肝损伤具有保护作用。

Protective effects of grape seed extracts against the acute chemical liver injury in mice

Objective:To study the protective effects of grape seed extracts(GSE) against the acute chemical liver injury in mice and research its possible mechanism for offering scientific basis of its health function.Methods:Randomly distribute 60 male Kunming mice to control group,acute chemical liver injury model group,DDB group(150 mg/kg) and low,middle,high dose groups of GSE(50,250,500 mg/kg).Determination and comparing of liver and spleen index between groups,using Reitman-Frankel to assay serum ALT and AST levels,xanthine oxidase to assay liver tissue SOD activities and thiobarburic acid to assay MDA content in liver tissue,and observing histopathological change of liver.Results:Treatment with different dose GSE could significantly increase serum ALT,AST levels(P<0.01) and liver tissue SOD activities(P<0.01),decrease MDA content in liver tissue(P<0.01).Histopathological observations showed that GSE could ameliorate the liver injury in different degree.Conclusions:GSE showed protective effects on the acute chemical liver injury induce by carbon tetrachloride in mice.