双色银染原位杂交技术检测胃癌HER2基因扩增及临床意义

目的 探讨双色银染原位杂交技术（dual-color silver-enhanced in-situ hybridization,DSISH）检测胃癌人类表皮生长因子受体2（human epidermalgrowth factor receptor-2,HER2）基因扩增的可行性及其与临床病理学特征的关系.方法 应用DSISH技术检测230例患者胃癌切除组织中HER2基因扩增状态,并进行相关统计学分析.结果 DSISH检测230份胃癌标本中43例存在HER2基因扩增,HER2基因扩增率为18.7％.HER2基因扩增与胃癌的分化程度、淋巴结转移有关（P均＜0.05）,与患者年龄、性别、肿瘤发生部位、浸润深度、神经以及脉管侵犯无关（P均＞ 0.05）.结论 DSISH技术检测胃癌HER2基因扩增状态具有可行性;HER2基因扩增可以反应胃癌生物学行为.

Dual-color silver-enhanced in-situ hybridization for determination of HER2 gene amplification in gastric carcinoma and clinical significance

Objective To evaluate the application of dual-color silver-enhanced in-situ hybridization （DSISH） in analysis of human epidermalgrowth factor receptor-2 （HER2） gene status and investigate the correlation between HER2 gene status and the clinical pathological parameters in gastric cancer. Methods HER2 gene status was detected in 230 cases of gastric cancer tissue by using automated DSISH and the results were analyzed statistically. Results Among 230 cases of gastric cancer tested by DSISH, 43 eases were positive, and all gene amplification rate was 18.7%. HER2 gene amplification were significantly correlated with degree of differentiation of gastric cancer and lymph node metastasis （Pall〈 0.05）, but not correlated with patient＇s age, gender, site of gastric cancer, the depth of tumor invasion and nerve, vascular invasion （Pall〉 0.05）. Conclusion Automated DSISH can be used to detect the HER2 gene status. HER2 gene amplification may reflect biological behavior of gastric cancer.