缬沙坦对血管紧张素Ⅱ诱导的牛主动脉血管平滑肌增殖及迁移的影响

目的 :观察血管紧张素Ⅱ (AngⅡ )及缬沙坦对培养的牛主动脉血管平滑肌细胞 (VSMC)增殖、迁移的作用。方法 :取新生小牛胸主动脉采用贴块法培养平滑肌细胞 ,分成对照组、AngⅡ组、缬沙坦组、缬沙坦加AngⅡ组 ,干预后分别测定3 H 胸腺嘧啶 (3 H TdR)掺入量和计数迁移的细胞。结果 :AngⅡ (1μmol/L)作用 2 4h能使VSMC的3 H TdR的掺入量、细胞迁移数较对照组增多 ;与AngⅡ组相比 ,AngⅡ加用缬沙坦 (10 0 μmol/L)使3 H TdR的掺入量与细胞迁移数明显减少 ;与对照组相比 ,单用缬沙坦使3 H TdR的掺入量亦明显减少。结论 :AngⅡ对VSMC有促增殖、迁移作用 ,能被缬沙坦拮抗 ,而且缬沙坦在没有AngⅡ作用时亦能单独发挥抗增殖作用

Effect of valsartan on cell proliferation and cell migration induced by angiotensin Ⅱ in cultured bovine thoracic vascular smooth muscle cell

Objective: To investigate the effects of valsartan on cell proliferation and cell migration induced by Ang Ⅱ in cultured bovine thoracic vascular smooth muscle cells(VSMC).Methods: VSMC from thoracic aorta of new born calf were isolated by outgrowth of the explant method,the experiment was divided into control group,Ang Ⅱ group,valsartan group,valsartan and Ang Ⅱ group,3H-TdR incorporation and cell migration were measured.Results: 24 h exposure to Ang Ⅱ (1μmol/L) caused an increase in 3H-TdR incorporation,cell migration number also increased.The proliferation and migration was inhibited by valsartan (100 μmol/L).Compared with control group,valsartan group showed a significant decrease of 3H-TdR incorporation.Conclusion: Vlsartan antagonized the proliferation and the migration of VSMC induced by Ang Ⅱ.Valsartan inhibited the proliferation of VSMC by itself without the action of Ang Ⅱ