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| 甜叶苷R1水解产物苷元的分离鉴定与抗帕金森病药效研究 | |
| 引用本文： | 陈攀,朱小南,杨奕,汪雪兰,陈汝筑. 甜叶苷R1水解产物苷元的分离鉴定与抗帕金森病药效研究[J]. 中药新药与临床药理, 2011, 22(3) |
| 作者姓名： | 陈攀 朱小南 杨奕 汪雪兰 陈汝筑 |
| 作者单位： | 中山大学中山医学院药理教研室,广州,510080 |
| 基金项目： | 广东省高等学校中药有效性与安令性重点实验室开放基金资助项目 |
| 摘 要： | 目的 研究甜叶苷R1(Suavissimoside R1)的水解产物苷元是否具有抗帕金森病(Parkinson's disease,PD)药理作用.方法 先用体外化学方法碱性水解甜叶苷R1,其水解产物用硅胶柱层析分离,将含量最多的化合物纯化收集后经核磁共振13C谱、1H谱、EI-MS谱以及熔点等数据分析后确定该物质结构为甜叶苷R1的苷元.在1-甲基-4-苯基吡啶离子(MPP+)处理的大鼠原代培养中脑多巴胺(DA)能神经元凋亡的PD细胞模型和1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)处理的小鼠PD动物模型上检测甜叶苷R1的苷元是否具有保护多巴胺能神经元的药理作用.结果 甜叶苷R1水解分离得到苷元,苷元纯度为95.8%.甜叶苷Rl在100 μmol·L-1和300 μmol·L-1浓度时对MPP+处理的大鼠原代培养中脑多巴胺能神经元有明显的保护作用.甜叶苷R1在0.75mg·kg-1剂量时能够明显提升MPTP处理的小鼠纹状体DA含量.甜叶苷R1的苷元则没有显示出明显的保护作用.结论 甜叶苷R1,而不是其苷元具有抗PD作用. |
| 关 键 词： | 茅莓 甜叶苷R1 苷元 帕金森病 多巴胺 |
Isolation and Identification of Aglycone Obtained from Hydrolysate of Suavissimoside R1 and Its Pharmacological Study of Anti-parkinson' s Disease |
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| CHEN Pan,ZHU Xiaonan,YANG Yi,WANG Xuelan,CHEN Ruzhu. Isolation and Identification of Aglycone Obtained from Hydrolysate of Suavissimoside R1 and Its Pharmacological Study of Anti-parkinson' s Disease[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2011, 22(3) | |
| Authors: | CHEN Pan ZHU Xiaonan YANG Yi WANG Xuelan CHEN Ruzhu |
| Affiliation: | CHEN Pan,ZHU Xiaonan,YANG Yi,WANG Xuelan,CHEN Ruzhu(Department of Pharmacology,Zhongshan Medical School,Sun Yat-sen University,Guangzhou 510080,China) |
| Abstract: | Objective To study the Pharmacological effect of the aglycone of suavissimoside R1 hydrolysate on Parkinson's disease.Methods Purified aglycone was obtained through silica gel column chromatography after hydrolysis of suavissimoside R1 with treatment of 3 % NaOH,and the aglycone structure was identified by mass spectrometry(MS),nuclear magnetic resonance(NMR),ultraviolet spectrometry(UV) and melting point(mp).The neuroprotective effects of suavissimoside R1 and the aglycone were investigated on the rat midb... |
| Keywords: | Rubus parvifolius L. Suavissimoside R1 Aglycone Parkinson's disease Dopamine |
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