| Expression pattern of neuregulin-1 type Ⅲ during the development of the peripheral nervous system |
| |
| 作者姓名： | Liang-liang Huang Zhong-yang Liu Jing-hui Huang Zhuo-jing Luo |
| |
| 作者单位： | Department of Orthopedics,Xijing Hospital,the Fourth Military Medical University of Chinese PLA |
| |
| 基金项目： | supported by grants from the National Program on Key Basic Research Project of China(973 Program),No.2014CB542206;the National Natural Science Foundation of China,No.81201389,30973052;Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China,No.IRT13051 |
| |
| 摘 要： | Neuregulin-1 type III is a key regulator in Schwann cell proliferation, committing to a myelinating fate and regulating myelin sheath thickness. However, the expression pattern of neuregulin-1 type III in the peripheral nervous system during developmental periods(such as the premyelinating stage, myelinating stage and postmyelinating stage) has rarely been studied. In this study, dorsal root ganglia were isolated from rats between postnatal day 1 and postnatal day 56. The expression pattern of neuregulin-1 type III in dorsal root ganglia neurons at various developmental stages were compared by quantitative real-time polymerase chain reaction, western blot assay and immunofluorescent staining. The expression of neuregulin-1 type III m RNA reached its peak at postnatal day 3 and then stabilized at a relative high expression level from postnatal day 3 to postnatal day 56. The expression of neuregulin-1 type III protein increased gradually from postnatal day 1, reached a peak at postnatal day 28, and then decreased at postnatal day 56. Immunofluorescent staining results showed a similar tendency to western blot assay results. Experimental findings indicate that the expression of neuregulin-1 type III in rat dorsal root ganglion was increased during the premyelinating(from postnatal day 2 to postnatal day 5) and myelinating stage(from postnatal day 5 to postnatal day 10), but remained at a high level in the postmyelinating stage(after postnatal day 10).
|
| 关 键 词： | nerve regeneration Schwann cells dorsal root ganglia myelin sheath neuregulin-1 type III peripheral nervous system quantitative real-time polymerase chain reaction western blot immunofluorescent staining postmyelinating rats NSFC grants neural regeneration |
Expression pattern of neuregulin-1 type Ⅲ during the development of the peripheral nervous system |
| |
| Liang-liang Huang,Zhong-yang Liu,Jing-hui Huang,Zhuo-jing Luo.Expression pattern of neuregulin-1 type Ⅲ during the development of the peripheral nervous system[J].Neural Regeneration Research,2015,10(1):65-70. |
| |
| Authors: | Liang-liang Huang;Zhong-yang Liu;Jing-hui Huang;Zhuo-jing Luo; |
| |
| Institution: | Liang-liang Huang;Zhong-yang Liu;Jing-hui Huang;Zhuo-jing Luo;Department of Orthopedics,Xijing Hospital,the Fourth Military Medical University of Chinese PLA; |
| |
| Abstract: | Neuregulin-1 type III is a key regulator in Schwann cell proliferation, committing to a myelinating fate and regulating myelin sheath thickness. However, the expression pattern of neuregulin-1 type III in the peripheral nervous system during developmental periods(such as the premyelinating stage, myelinating stage and postmyelinating stage) has rarely been studied. In this study, dorsal root ganglia were isolated from rats between postnatal day 1 and postnatal day 56. The expression pattern of neuregulin-1 type III in dorsal root ganglia neurons at various developmental stages were compared by quantitative real-time polymerase chain reaction, western blot assay and immunofluorescent staining. The expression of neuregulin-1 type III m RNA reached its peak at postnatal day 3 and then stabilized at a relative high expression level from postnatal day 3 to postnatal day 56. The expression of neuregulin-1 type III protein increased gradually from postnatal day 1, reached a peak at postnatal day 28, and then decreased at postnatal day 56. Immunofluorescent staining results showed a similar tendency to western blot assay results. Experimental findings indicate that the expression of neuregulin-1 type III in rat dorsal root ganglion was increased during the premyelinating(from postnatal day 2 to postnatal day 5) and myelinating stage(from postnatal day 5 to postnatal day 10), but remained at a high level in the postmyelinating stage(after postnatal day 10). |
| |
| Keywords: | nerve regeneration Schwann cells dorsal root ganglia myelin sheath neuregulin-1 type III peripheral nervous system quantitative real-time polymerase chain reaction western blot immunofluorescent staining postmyelinating rats NSFC grants neural regeneration |
| 本文献已被 CNKI 等数据库收录！ |
