CD40-CD40L在强直性脊柱炎患者外周血淋巴细胞上的表达

目的探讨共刺激分子CD40CD40L在强直性脊柱炎(AS)患者的外周血淋巴细胞亚群上的异常表达与免疫功能紊乱的关系。方法用流式细胞仪采用直接免疫荧光法测定30例AS患者和20例健康对照人外周血淋巴细胞表面标志CD3、CD4、CD8、CD19的表达情况,CD40L在CD4 T和CD8 T细胞上的表达及CD40在CD19 B细胞上的表达。用速率散射比浊法测定血清中免疫球蛋白IgG,IgA和IgM的水平。结果①AS患者CD3 、CD3 CD4 、CD19 细胞较正常对照组显著增高(P<0.05),CD3 CD8 细胞较正常对照组显著降低(P<0.05);②AS患者CD4 T细胞和CD8 T细胞上的CD40L、CD19 B细胞上CD40的表达都较对照组显著增高(P<0.05);③AS患者血清中2种免疫球蛋白IgG、IgA的水平均较对照组显著增高(P<0.05)。结论CD40CD40L途径在AS免疫功能紊乱中起了重要作用。

Expression of CD40-CD40L on peripheral blood lymphocytes of patients with Ankylosing spondylitis

Objective To investigate the relationship between abnormal expression of costimulator molecule CD40-CD40L on lymphocyte subpopulation and the immune dysfunction in patients with ankylosing spondylitis (AS). Methods Samples from 30 AS patients and 20 healthy controls were analyzed by immunofluorescence and flow cytometry, for determining the expressions of CD3, CD4, CD8, and CD19 on lymphocytes,CD40L on CD4 T cells or CD8 T cells,and CD40 on CD19 B cells. IgG, IgA, and IgM in serum were determined by rate nephelometry. Results As compared with controls, the CD3 T cells, CD3 CD4 T cells, and CD19 B cells in AS patients were significantly increased (P < 0.05), but the CD3 CD8 cells were significantly decreased (P < 0.05). The CD40L expressed on CD4 T cells and CD8 T cells, and the CD40 expressed on CD19 B cells in AS patients, were all significant higher than those in healthy controls (P < 0.05). The levels of IgG and IgA in serum of AS patients were significant higher than those in healthy controls (P < 0.05). Conclusion CD40-CD40L pathway may play an important role in immune dysfunction of AS patients.