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| 乌头碱羟基琥珀酰亚胺酯及其BSA结合物的制备 | |
| 引用本文： | 赵怀清,服部征雄. 乌头碱羟基琥珀酰亚胺酯及其BSA结合物的制备[J]. 沈阳药科大学学报, 2000, 17(1): 26-29 |
| 作者姓名： | 赵怀清 服部征雄 |
| 作者单位： | 1. 沈阳药科大学基础部,沈阳,110015 2. 日本富山医科药科大学和汉药研究所,930-01,日本富山 |
| 摘 要： | 为建立乌头碱酶免疫测定法，合成了乌头碱衍生物，用羟基琥珀酰亚胺酯的方法制备半抗原（ 活性酯） ，并用其半抗原与ＢＳＡ（Ｂｏｖｉｎｅ Ｓｅｒｕｍ Ａｌｂｕｍｉｎ） 在有机溶媒／ 磷酸缓冲液（ｐＨ７－３） 的条件下制备了抗原性ＢＳＡ 结合物．结果显示，这种活性酯的方法可用于乌头碱酶免疫测定法中与ＢＳＡ 或酶交联的有效方法． |
| 关 键 词： | 乌头碱 半抗原 N-羟基琥珀酰亚胺酯 抗原性BSA结合物 合成 |
| 修稿时间： | 1999-04-05 |
The Preparation of Aconitine N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum Albumin |
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| Zhao Huaiqing,Hattori Masao. The Preparation of Aconitine N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum Albumin[J]. Journal of Shenyang Pharmaceutical University, 2000, 17(1): 26-29 | |
| Authors: | Zhao Huaiqing Hattori Masao |
| Abstract: | In order to establish the enzyme immunoassay of aconitine,aconitine derivatives were synthesized,which is called the chemical bridge.The preparation of the hapten of aconitine was carried out by the N hydroxysuccinimide esters method.These activated esters were covalently linked to BSA(Bovine Serum Albunin)in an organic solvent/phosphate buffer(pH 7 3) employing 1∶30 molar ratio of the aconitine to protein.The results indicated that the activated ester method would be useful for enzyme labeling in enzyme immunoassay of aconitine in respect of simplicity and reproducibility. |
| Keywords: | aconitine hapten N hydroxysuccinimide ester immunogenic conjugate synthesis |
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