四氧嘧啶诱导兔1型糖尿病模型的实验研究

目的应用不同剂量四氧嘧啶（ALX）诱导兔糖尿病模型,探索一种安全、稳定的糖尿病模型建立方法。方法健康新西兰兔随机分为A、B 2组。2组给ALX前均禁食12 h,A组按150 mg/kg体重1次给药;B组为2次给药法组,第1次按80 mg/kg体重,24 h后按120 mg/kg体重给药。14 d后经耳缘静脉测空腹血糖值,以空腹血糖〉16 mmol/L作为造模成功的标准。比较2组的病死率和成模率。结果A组病死率70%,成模率20%;B组病死率10%,成模率80%。两种不同的给药方法病死率和成模率比较差异均有统计学意义（P〈0.05）。结论应用ALX制备糖尿病模型,是一种经济、安全、可靠的方法,连续2次小剂量给药法优于1次性高剂量给药,降低了病死率,提高了成模率。

Experimental study on diabetic models in rabbits induced by alloxan

Objective To explore a safe, stable, low mortality method of inducing diabetic model by alloxan in rabbits. Methods The healthy New Zealand rabbits were randomly divided into 2 groups, and they were fasted 12 hours before injection of alloxan. The rabbits in group A were directly given ALX 150 mg/kg once, and the rabbits in group B were given ALX twice, 80 mg/kg of ALX was intravenously injected firstly, 24 hours later, 120 mg/kg of ALX was given again. 14 days later, fasting blood glucose was detected, and the standard of diabetic model was fasting blood glucose 〉 16 mmol/L. The death rate and model-forming rate were compared between the two groups. Results In group A, death rate was 70%, and model-forming rate was 20%. In group B, death rate was 10%, and model-forming rate was 80%. There were significant differences in death rate and model-forming rate between the two different medications （P 〈 0.05） . Conclusinn The diabetic animal model induced by ALX is a safe, economic, reliable method. The twice administration method is superior to that once administration method with a higher model-forming rate and lower death rate.