加味补阳还五汤结合头穴丛刺对缺血性中风大鼠NCAM阳性表达的影响

目的：对比研究加味补阳还五汤方结合头穴丛刺法（下文简称针药法）对气虚血瘀型缺血性中风大鼠NCAM阳性表达的影响,为临床治疗此型中风提供更加有效的中医诊疗方案.方法：选取健康大鼠进行气虚血瘀症侯造模,成功后给予DiL染料预标记,随机分组后,48h后进行MCAO造模,制备气虚血瘀型缺血性中风大鼠模型,模型建立成功后,分别采用头穴丛刺法、补阳还五汤原方、加味补阳还五汤、针药法分组治疗,于不同时间点以原位杂交法检测指标,进行对比研究,观察各法对模型大鼠NCAM阳性表达的影响.结果：治疗后原位杂交检测阳性细胞计数,各时间点各治疗组大鼠均有明显变化,针药组效果最佳,具有极显著性差异（P＜0.01）.结论：各种治疗方法对模型大鼠NCAM阳性表达均具有一定的促进作用,多种方法比较,针药结合应用效果最佳.

Effect of Flavored Buyang Huanwu Decoction Combined with Cluster Needling of Scalp Point on the Expression of NCA MmRNA of Ischemic Stroke Rats

Objective ： To study the effect of cluster needling of scalp point combined with the prescription of fla- vored Buyang Huanwu decoction （hereinafter referred to as acupuncture plus drug method） on the NCAM of the rat models with Qi deficiency and blood stasis in ischemic apoplexy, and to provide optimized clinical schemes in TCM treatment. Methods： The rat models with Qi deficiency and blood stasis, made from healthy ones, were pre - labeled. Assigned randomly into groups, MCAO models were made after 48 hours, with rat models of Qi deficiency and blood stasis in ischemic apoplexy. After the models were made, the randomly - assigned groups were given different treatments to observe the effect of NCAM on rat models： cluster needling of scalp point, the original Buyang Huanwu decoction, flavored Buyang Huanwu decoction, and acupuncture plus drug method. The comparison of the results would be based on test index such as ISH （ in situ hybridiza- tion）. Results： There was a significant difference in the positive cell counts by ISH in every group in differenttime point after treatment, in which flavored decoction group was superior to original one, and the effect of ac- upuncture plus drug group was the most obvious compared with others （ P 〈 O. Ol ）. Conclusion ： The different treatment methods mentioned in the paper have the positive effect on rat models to some degree in their recov- ery of ISH. The effect of acupuncture plus drug method is the most obvious compared with others, and the effect of flavored Buyang Huanwu decoction is superior to original one.