| Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele |
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| 引用本文： | P. Tzvetkova Wei-jie ZHU Jing LI D. Tzvetkov. Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele[J]. 生殖与避孕(英文版), 2007, 18(1): 19-24. DOI: 10.1016/S1001-7844(07)60003-0 |
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| 作者姓名： | P. Tzvetkova Wei-jie ZHU Jing LI D. Tzvetkov |
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| 作者单位： | [1]Institute of Experimental Morphology and Anthropology with Museum, Bulgarian Academy of Science, Sofia, Bulgaria [2]Center for Reproductive hnmunology Research, Jinan University, Guangzhou 510632, China [3]Medical College, Jinan University, Guangzhou 510632, China [4]Department of Urology, Medical University, Sofia, Bulgaria |
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| 摘 要： | Objective To determine the sperm acrosome integrity of samples from infertile men with varicocele. Methods Forty-nine infertile men with varicocele were divided into three groups according to the grade of varicocele. Group A （grade Ⅰ）, B （grade Ⅱ）, and C （grade Ⅲ） consisted of 15, 18, and 16 cases, respectively. Besides, 15 semen samples from normospermic donors were used as the control. The acrosome integrity of sperm was examined with fluorescein-labeled Pisum sativum agglutinin. Acrosomal ultrastructure was observed with transmission electron microscopy. Results In three varicocele groups, most samples had high sperm abnormal morphology rates. There were significant differences in acrosome integrity rates between each varicocele group and the control （P〈0.01）. Group C had the lowest acrosome integrity rate among the three groups. Ultrastructural observation showed that acrosome malformations revealed acrosomal membranes defects, swelling, hypoplasia, and dissolution of the matrix. Conclusions Infertile men with varicocele had low level of acrosome integrity. Severe varicocele for infertile men might be associated with severe acrosomal defects. Evaluating sperm acrosome should aid the understanding of the sperm structural state and benefit the treatment for infertile men.
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| 关 键 词： | 精索静脉曲张 男性不育 精子 顶体完整性 评定 |
| 收稿时间： | 2006-11-28 |
Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele |
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| P.Tzvetkova,Wei-jie ZHU,Jing LI,D.Tzvetkov. Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele[J]. Journal of Reproduction and Contraception, 2007, 18(1): 19-24. DOI: 10.1016/S1001-7844(07)60003-0 |
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| Authors: | P.Tzvetkova Wei-jie ZHU Jing LI D.Tzvetkov |
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| Affiliation: | 1. Wildlife Toxicology Group, National Wildlife Research Institute (Instituto de Investigación en Recursos Cinegéticos, IREC), UCLM-CSIC-JCCM, Ronda de Toledo s/n, 13071 Ciudad Real, Spain;2. SaBio, National Wildlife Research Institute (Instituto de Investigación en Recursos Cinegéticos, IREC), UCLM-CSIC-JCCM, 02071 Albacete, Spain |
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| Abstract: | Objective To determine the sperm acrosome integrity of samples from infertile men with varicocele.Methods Forty-nine infertile men with varicocele were divided into three groups according to the grade of varicocele.Group A(grade I),B(grade II),and C(grade III)consisted of 15,18,and 16 cases,respectively.Besides,15 semen samples from normospermic donors were used as the control.The acrosome integrity of sperm was examined with fluorescein-labeled Pisum sativum agglutinin.Acrosomal ultrastructure was observed with transmission electron microscopy.Results In three varicocele groups,most samples had high sperm abnormal morphol-ogy rates.There were significant differences in acrosome integrity rates between each varicocele group and the control(P<0.01).Group C had the lowest acrosome integrity rate among the three groups.Ultrastructural observation showed that acrosome mal-formations revealed acrosomal membranes defects,swelling,hypoplasia,and dissolu-tion of the matrix.Conclusions Infertile men with varicocele had low level of acrosome integrity.Severe varicocele for infertile men might be associated with severe acrosomal defects.Evalu-ating sperm acrosome should aid the understanding of the sperm structural state and benefit the treatment for infertile men. |
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| Keywords: | varicocele acrosome sperm infertility |
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