XIAP和survivin在原发性肝细胞癌中的表达及其相关性

目的 探讨X染色体连锁的凋亡抑制蛋白(XIAP)和存活素(survivin)在原发性肝细胞癌(简称肝癌)组织中的表达情况并探讨二者之间的关系.方法 采用免疫组织化学法检测38例肝癌组织和16例癌旁组织中XIAP和survivin的表达情况并对其进行评分.结果 肝癌组织中XIAP和survivin表达阳性率分别为81.6％(31/38)和78.9％ (30/38),16例癌旁组织中表达阳性率分别为12.5％ (2/16)和6.3％(1/16),二者在肝癌组织及癌旁组织中表达阳性率的差异均有统计学意义(P＜0.001).XIAP在高、中及低分化肝癌组织中的表达评分分别为(2.91±1.31)分、(9.27±3.25)分和(13.08±2.26)分(F=118.948,P＜0.001)；survivin在高、中及低分化肝癌组织中的表达积分分别为(4.85±1.83)分、(11.08±3.72)分和(13.38±1.76)分(F=72.202,P＜0.001).两者的表达强度与肿瘤大小均无关(P＞0.05).XIAP和survivin在肝癌组织中的表达强度呈正相关(r=0.764,P＜0.001).结论 XIAP和survivin在肝癌中的表达强度均与肿瘤的分化程度有关,二者的表达强度在肝癌组织中的表达存在相关性,可能在肝癌的恶性转化以及耐药方面发挥重要作用.

Expression and Correlation of XIAP and Survivin in Primary Hepatocellular Carcinoma

Objective To investigate the expressions of X-linked inhibitor of apoptosis protein(XIAP)and survivin in primary hepatocellular carcinoma tissues,and to explore the relationship between them.Methods The expressions of XIAP and survivin protein in 38 primary hepatocellular carcinoma tissues and 16 paracancerous tissues were detected by using immunohistochemistry and the expressions were scored.Results The positive expression rate of XIAP and survivin in primary hepatocellular carcinoma tissues was 81.6%(31/38)and 78.9%(30/38),respctively(P< 0.001),and in paracancerous tissues was 12.5%(2/16)and 6.3%(1/16),respectively(P<0.001).The score of XIAP expression in the well,middle,and low differentiated tissues of primary hepatocellular carcinoma was(2.91±1.31),(9.27±3.25),and(13.08±2.26)score,respectively(F=118.948,P<0.001),and the score of survivin expression was(4.85±1.83),(11.08±3.72),and(13.38±1.76)score,respectively(F=72.202,P<0.001).They both significantly correlated with the histological grade,but not with the size of tumor(P>0.05).There was significantly correlation between the expression intensity of XIAP and survivin in primary hepatocellular carcinoma tissues(r=0.764,P< 0.001).Conclusions The expression intensity of XIAP and survivin in primary hepatocellular carcinoma tissues are both related with the differentiation of primary hepatocellular carcinoma.The expression intensity of XIAP is related with the survivin,and they may play an important role in the tumor progression and chemical resistances.