血必净对脓毒症大鼠肠黏膜屏障及巨噬细胞抗体表达的作用

目的 观察血必净对脓毒症大鼠肠黏膜屏障和巨噬细胞抗体表达的变化的影响作用,探讨其在脓毒症发病中的作用机制及意义.方法 Wistar大鼠150只,分成脓毒症组、血必净干预组和假手术组.采用肓肠结扎穿孔术建立脓毒症大鼠模型,模型标准是发热、呼吸频率增加、心率加快和白细胞总数改变.血必净组大鼠术前12 h、术后腹腔内注射4 mL/kg血必净,1次/12 h,共3 d;其余两组大鼠同时注射相同体积的生理盐水.利用图像分析系统检测三组大鼠肠黏膜病理变化和巨噬细胞的表达水平,并运用等级资料秩和检验和方差分析进行统计学分析.结果 假手术组大鼠小肠黏膜多为基本正常黏膜,12 h,24 h,48 h,72 h脓毒症组、血必净组大鼠肠道黏膜出现病变;脓毒症组病变较血必净组更为严重,差异具有统计学意义(H=19.732,P<0.01).在0 h三组小肠黏膜巨噬细胞抗体的表达数基本一致,12 h,24 h,48 h,72 h脓毒症组和血必净组间差异具有统计学意义,且均较C组变化差异具有统计学意义(F=560.13,P<0.05).结论 脓毒症大鼠肠道黏膜机械屏障和免疫屏障均有不同程度的损害,血必净注射液可部分保护肠道黏膜机械屏障和免疫屏障.

The effects of Xuebijing injection on intestinal tract mucosa barrier and on the expression of macrophage antibody in rats with sepsis

Objective To study the effect of Xuebijing injection on the change of intestinal tract mucosa barriers and the expression of macrophage antihody in rats with sepsis. Method Totally 150 Wistar rats were ran-domly divided into three groups: sham group, sepsis group, and Xuebijing group. Sepsis models were estabhshed in rats by cecal ligation and puncture. The success standards of sepsis included fever, the increase of respiration rate,increased heart rate and change in leukocyte count. At 12 hours before operation, and after operation the rats in the Xuebijing group were injected with 4 mL/kg Xuebijing (once every 12 hours for 3 days), and the rats in the others two groups were injected with the same volume of saline, the image analytical system were used to detect the pathological change of intestinal tract mucosa harriers and the expression of macrophage antibody in three groups. All the data were analyzed by rank-sum test and variance analysis(F test). Results Almost all the mucous mem-brahe of small intestine in sham groups were normal. The mucous membrane lesions of small intestine developed in sepsis and Xuebijing groups after 12,24,48,72 hours, and the lesion was severer in sepsis group than that in the Xuebijing group (H=19.732, P<0.01). There were no significant differences on the expression of macrophage antibody in mucous membrane of small intestine between three groups at 3 hours. But at 12, 24, 48 and 72 hours, the expression of macmphage antibody of sepsis and Xnebijing groups increased with time (F=560.13, P< 0.05). Conclusions At the early period of sepsis, intestinal tract mucosa barriers develops varying degrees of damage, and Xnebijing can partly protect intestinal tract mucosa barriers.