乌司他丁与高渗盐水单独或联合预处理对大鼠脑缺血再灌注的影响

【目的 观察和比较乌司他丁（Urinary Trypsin Inhibitor，UTI）与高渗盐水（Hypertonic Saline，HTS）单独或联合预处理对大鼠脑缺血再灌注的影响。方法 SD大鼠60只建立MCAO模型，随机分为4组，均在缺血前10 min预处理：对照组（C组），0.9%NaCl4 ml/㎏ iv；HTS组（H组），7.5%NaCl4 ml/㎏ iv；UTI组（U组），UTI10万U/㎏ iv（以1.5 mlNS溶解）； UTI+HTS组（U+H组），UTI10万U/㎏+7.5%NaCl4 ml/㎏ iv。再灌注22 h后取脑组织切片观察并检测S100β蛋白的含量及梗死体积。结果 预处理组梗死体积及S100β蛋白显著低于C组（P＜0.01），U组梗死体积及S100β蛋白显著高于H组而显著低于U+H组（P＜0.01）。结论 在大鼠脑缺血再灌注，UTI与HTS联合预处理较二者单独应用可提供更好的脑保护作用。 【关键词】 脑缺血再灌注 乌司他丁 高渗盐水 S100β蛋白 梗死体积

The effect of preconditioning with ulinastatin or hypertonic saline alone or together on cerebral ischemia/reperfusion in rats

Abstract】 Objective To observe and compare the effect of preconditioning with ulinastatin or hypertonic saline alone or together on cerebral ischemia/reperfusion in rats. Methods Animals are randomly assigned to receive normal saline4ml/㎏(C group), ulinastatin100000U/㎏(U group)，7.5%NaCl4ml/㎏(H group) or ulinastatin &7.5%NaCl(U&H group) intravenous respectively 10 min before MCAO of 2h, all animals sacrificed after reperfusion of 22h, infarction volume and S100βdetermined .Results Animals in C group get the largest infarction volume and highest S100β, infarction volume and S100βof U group is higher than those of U&H group while lower than H group. Conclusion Preconditonig with ulinastatin and 7.5%NaCl together has the best cerebroprotective effect, shown as the smallest infarction volume and lowest S100β.