大鼠急性心肌缺血/再灌注模型改良方法与传统方法的比较

目的:建立大鼠急性心肌缺血/再灌注模型的改良方法与传统方法并进行比较。方法:将60只SD大鼠随机分为两组,每组30只;一组采用改良方法建立模型,即气体麻醉,以气体麻醉面罩通气,短时间开胸;另一组采用传统方法建立模型,即腹腔麻醉,气管插管,长时间开胸。比较两种方法的手术时间、手术成功率、术中血氧饱和度、术后存活率、心肌梗死面积,以及术后大鼠的生活状况。结果:与传统方法组相比,改良方法组大鼠术中存活率升高[(93.3±4.6)%vs.(72.4±8.4)%,P0.05]、开胸时间缩短为[(6.5±2.0)minvs.(44.9±2.7)min,P0.01],开胸前后平均血氧饱和度增加[(96.3±0.8)%vs.(90.9±2.1)%,P0.05],术后存活率提高(82.83%vs.58.3%,P0.01)。另外改良方法组大鼠术后恢复正常行为活动需要的时间较短,但两组大鼠心肌梗死的面积[(33.5±2.2)%vs.(35.0±3.0)%]无统计学意义。结论:改良方法具有简单、高效及可独立操作的优点,能有效提高建立大鼠急性心肌缺血/再灌注模型的成功率。

Comparison between conventional and modified methods of acute myocardial ischemia/reperfusion models

AIM: To develop and compare the efficacy of a modified vs. conventional rat model of acute myocardial ischemia/reperfusion. METHODS: Sixty Sprague Dawley (SD) rats were randomly divided into two groups: a modified group (respiratory mask support and short-time chest opening) and a conventional group (tracheal intubation and long-time chest opening). Surgical time, surgical success rate, survival rate, and infarct size were investigated, and the postoperative survival rate of the rats was observed. RESULTS: During the perioperative period, the surgical success rate was higher in the modified model ［(93.3±4.6)% vs. (72.4±8.4)%, P<0.05］. Chest-opening time was shorter in the modified group vs. the conventional model ［(6.5±2.0) min vs. (44.9±2.7) min, P<0.01］. Postoperative resumption of normal behaviors and activities was quicker in the modified group, with a statistically significant higher survival rate compared with that in the conventional group (82.83% vs. 58.3%, P<0.001). Triphenyltetrazolium chloride staining showed no significant difference in infarct size between groups ［(33.5±2.2)% vs. (35.0±3.0)%］. CONCLUSION: The modified method offers the advantages of simplicity, efficiency and independent operation, which enhances the success rate of establishing the acute rat myocardial ischemia/reperfusion model.