白细胞介素10基因单核苷酸多态性与小儿哮喘易感性的相关性研究

目的：探讨白细胞介素10（IL-10）基因3个单核苷酸多态性位点（rs1800896、rs3021097和rs3024492）与哮喘遗传易感性的相关性。方法：采用Sequenom MassARRAY-IPLEX SNP检测系统,分别对湖北省黄石地区汉族人群中200例正常健康者（对照组）和189例哮喘患者组（病例组）IL-10 3个单核苷酸多态性位点（SNP）（rs1800896、rs3021097和rs3024492）进行基因分型并分析两组间分布情况。用Hardy-Weinberg平衡对位点的基因型分布进行检验后,用χ2检验统计分析病例组和对照组基因型和等位基因的频率;采用Logistic回归模型进行计算,消除性别年龄因素对统计的影响,寻找出在病例组和对照组之间存在显著性差异的SNP位点,从而揭露IL-10多态性与哮喘之间的关联性。结果：①rs1800896位点存在G/G、G/A、A/A 3种基因型,其在病例组的分布频率为4.2%、57.1%、38.7%,在对照组的分布频率为0%、19.5%、80.5%,两组之间差异有统计学意义（P=0.001）;②rs3021097位点存在T/T、C/T和C/C 3种基因型,在病例组的分布频率为49.7%、40.8%和9.5%,在对照组的分布频率为46.5%、43.5%和10%,两组之间差异有显著统计学意义（P=0.45）;③rs3024492位点在汉族人群中只存在T/A和A/A 2种基因型,其在病例组的分布频率为4.8%和95.2%,在对照组的分布频率为5.5%和94.5%。两组之间有差异,但无统计学意义（P=0.7）。结论：在湖北黄石地区人群中,IL-10基因多态性与小儿哮喘易感性相关。其中rs1800896和rs3021097与小儿哮喘的易感性显著相关,而rs3024492与小儿哮喘的易感性不相关。

Polymorphisms and Susceptibility to Asthma in Children Study of Interleukin-10 Gene Single Nucleotide

Objective ： To investigate the intedeukin-10 （IL-10） gene three single nucleotide polymorphisms （ rs1800896, rs3021097 and rs3024492） and genetic susceptibility to asthma. Methods：Sequenom MassARRAY-IPLEX SNP detection system, respectively, IL-10 3 single nucleotide polymorphisms in Huangshi, Hubei Province Han population 200 cases and 189 control group patients with asthma group （SNP） （ rs1800896, rs3021097 and rs3024492 ） were genotyped and analyzed the distribution between the two groups. After testing with Hardy-Weinberg equilibrium genotype distribution of sites, using the X2 test cases and statistical analysis of genotype and allele frequencies of the control group ; Logistic regression models were applied to eliminate the effects of sex and age factors on statistics Find out the existence of significant differences between the case and control groups SNP loci, thus exposing IL-10 association between polymorphisms and asthma. Results ：（1） rs1800896 locus G/G, G/A, A/A 3 genotypes, its frequency distribution asthma group was 4. 2%,57.1% ,38.7%, in the distribution of frequencies healthy control group was 0%, 19.5% ,80.5%, the difference between the two groups was statistically significant（P = 0.001 ） ;presence （2） rs3021097 locus T/T, C/T and C/C 3 genotype, allele in the asthmatic group was 49.7 % ,40.8 % and 9.5 % in the healthy control group,the frequency distribution was 46.5 % ,43.5 % and 10 %. There are differences between the two groups was statistically significant（P =0.45 ） ;@ rs302d492 loci in Han population there is only T/A and A/A 2 genotypes,its frequency distribution asthma group was 4.8% and 95.2% in the frequency distribution of healthy control group was 5.5% and 94.5%. There was no significant difference between the two groups （P = 0.7）. Conclusion：China Hubei Huangshi area population, and IL-10 gene polymorphism in children with asthma susceptibility. Which rs1800896 and rs3021097 with susceptibility to asthma in children was significantly associ