5-aza-2'-deoxycytidine therapy in patients with acute leukemia inhibits DNA methylation |
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| Authors: | R L Momparler J Bouchard N Onetto G E Rivard |
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| Affiliation: | Département de Pharmacologie, Université de Montréal and Centre de Recherche Pédiatrique, Hôpital, Ste-Justine, Montreal, Quebec, Canada |
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| Abstract: | ![]()
The effect of 5-aza-2'-deoxycytidine (5-AZA-dCyd) on methylation of DNA in blood leukemic cells from patients with lymphoid and myeloid leukemia was investigated. After treatment with continuous infusion of 5-AZA-dCyd (1.0 mg/kg/h) the blood leukemic cells were isolated and incubated in vitro with [6-3H]deoxycytidine and the incorporation of radioactivity into 5-methylcytosine and cytosine of DNA determined. 5-AZA-dCyd produced greater than 70% inhibition of DNA methylation in the leukemic cells. The antileukemic action of 5-AZA-dCyd may be related to the inhibition of DNA methylation. |
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| Keywords: | DNA methylation differentiation therapy 5-AZA-2′-deoxycytidine 5-aza-2′-deoxycytidine white blood cell count |
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