光学相干断层扫描与3.0T MRI、超声法测量兔膝关节软骨厚度的比较

 目的 探索使用光学相干断层扫描技术(optical coherence tomography，OCT)无创测量兔膝关节软骨厚度的可行性。方法 选择标准化饲养成年雄性新西兰大白兔40只(80膝关节)，记号笔标记不同样本同一测量点，分别采用OCT技术、3.0 T MRI、超声测量方法，由专业技师对软骨厚度进行测量，比较OCT技术与其他两种方法测量兔膝关节软骨厚度的差异。结果 同一测量点OCT技术、3.0T MRI、超声法软骨厚度测量数值结果分别为(0.31±0.03)mm、(0.30±0.05)mm、(0.33±0.06)mm， OCT与超声(t=1.995，P=0.051 )、MRI(t=1.955，P=0.054)测量数据比较差异无统计学意义。单个样本同一测量点，OCT、3.0T MRI、超声测量平均结果获得时间分别为(2.5±0.3)min、(6.3±0.7)min、(3.3±0.6)min。三种测量方法的精确度分别为0.001 mm、0.01 mm、0.01 mm。结论 光学相干断层扫描技术测量兔膝关节软骨厚度具有精确、快速、重复性好、人为误差小的特点，值得在组织工程软骨修复关节软骨缺损动物实验中广泛推广。

Comparison of optical coherence tomography with 3.0 TMRI and ultrasonic pachymetry for thickness measurement of rabbit knee joint cartilage

Objective To verify the feasibility of optical coherence tomography(OCT) for thickness measurement of rabbit knee joint cartilage as a non-invasive measurement method via comparing with 3.0 T MRI and ultrasonic pachymetry. Methods 40 standardized culturea, adult and male New Zealand white rabbits were recruited for this study(80 knees). Ultrafine probe was used to mark measurement point. Using OCT, 3.0 T MRI and ultrasonic techniques, cartilage thickness data were obtained at the same point,respectively. The difference between OCT and the other two methods was compared. Results The numerical results of OCT, 3.0 T MRI, ultrasonic pachymetry measurement method of cartilage thickness were(0.31±0.03)mm,(0.30±0.05)mm,(0.33±0.06)mm,respectively, without statistically significant difference (OCT- ultrasonic t=1.995,P=0.051,OCT-MRI t=1.955,P=0.054). And the time required were(2.5±0.3)min、(6.3±0.7)min、(3.3±0.6)min, respectively. The accuracy of the three measuring methods were 0.001 mm, 0.01 mm and 0.01 mm. Conclusions Compared with 3.0 TMRI and ultrasonic pachymetry, OCT measurement of rabbit knee joint cartilage thickness has the advantages of precision, time-saving, good repeatability, low cost ,and human error small, worthy of animal experimental practice.