| 粒细胞集落刺激因子对小鼠急性肝衰竭的保护作用 |
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| 引用本文： | 徐旭雯,谭德明,鲁猛厚. 粒细胞集落刺激因子对小鼠急性肝衰竭的保护作用[J]. 中南大学学报(医学版), 2006, 31(4): 543-547 |
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| 作者姓名： | 徐旭雯 谭德明 鲁猛厚 |
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| 作者单位： | 中南大学湘雅医院传染病研究所,长沙,410008;中南大学湘雅医院传染病研究所,长沙,410008;中南大学湘雅医院传染病研究所,长沙,410008 |
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| 摘 要： | 目的：观察重组人粒细胞集落刺激因子（recombinant human granulocyte colony stimulating factor；rhG-CSF）对D-氨基半乳糖（D-galactosamine，D-GaIN）加脂多糖（lipopolysaccharide，LPS）联合诱导的小鼠急性肝衰竭的保护作用，并探讨其机制。方法：D-GAIN／LPS腹腔联合注射制造小鼠急性肝衰竭模型，治疗组小鼠于造模前4h，2h及造模同时予以G-CSF300μg／kg腹腔注射，观察小鼠24h存活率。造模后6h每组随机选择5只小鼠处死，观察肝组织损伤情况，用半定量逆转录一聚合酶链反应和TanonGis3、73软件分析各组小鼠肝组织中肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白介素-6（IL-6）和白介素-10（IL-10）的表达情况。结果：治疗组小鼠24h存活率明显高于对照组（P〈0.01），造模后6h治疗组小鼠肝组织损伤明显减轻（P〈0．05），肝组织中7YvF-α和IFN-γ的mRNA表达水平明显低于对照组（P〈0．01），IL-6和IL-10的mRNA表达水平明显高于对照组（P〈0．01）。结论：G-CSF对D-GalN／LPS所致的小鼠急性肝衰竭具有保护作用。
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| 关 键 词： | 急性肝衰竭 粒胞集落刺激因子 脂多糖 细胞因子 |
| 文章编号： | 1672-7347（2006）04-0543-05 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-08-29 |
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| XU Xu-wen,TAN De-ming,LU Meng-hou.
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| Authors: | XU Xu-wen TAN De-ming LU Meng-hou |
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| Affiliation: | Institute of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. |
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| Abstract: | OBJECTIVE: To evaluate the protective effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on acute hepatic failure induced by galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice, and to explore its mechanism. METHODS: The mice were intraperitoneally administered D-GalN (800 mg/kg) and LPS (10 microg/kg), and then were intraperitoneally injected either saline (the control group )or rhG-CSF at 300 microg/kg body weight (the therapy group) at 4 h, 2 h and 0 h before the D-GalN/LPS injection. The survival rate of the mice was estimated at 24 h after the D-GalN/LPS injection. The degree of hepatic injury was evaluated at 6 h after the D-GalN/LPS injection, and the levels of TNF-alpha, IFN-gamma, IL-6 and IL-10 mRNA were simultaneously measured by semiquantitative RT-PCR. RESULTS: The survival rate of the therapy group was significantly higher than that of the control group (68.4% vs 20%, P<0.01). As compared with the control group, the degree of liver injury in the therapy group significantly decreased (P<0.05), and the levels of TNF-alpha and IFN-gamma mRNA in the hepatic tissue also reduced remarkably (P<0.01, respectively), while the levels of IL-6 and IL-10 mRNA increased (P<0.01, respectively) at 6 h after the D-GalN/LPS injection. CONCLUSION: G-CSF can protect the mice from acute hepatic failure induced by D-GalN/LPS. |
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| Keywords: | acute hepatic failure granulocyte colony-stimulating factor lipopolysaccharide cytokine |
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