沙利度胺联合三氧化二砷抑制SMMC7721裸鼠移植瘤生长及毒副反应观察

目的：研究高低剂量沙利度胺（Tha）联合三氧化二砷（AsT）干预SMMC7721裸鼠移植瘤生长，检测移植瘤微血管密度（MVD）和核转录因子（NF—κB）表达，观察毒副反应。方法：取人肝癌细胞株SMMC7721接种于BALB／c裸鼠颈部皮下，随机分为7组。一周后按实验设计开始给药，每周称裸鼠体重，测移植瘤体积，连续给药4周。实验结束处死裸鼠，检测移植瘤MVD及NF-κB表达，鼠肺、肝、肾行HE染色，鼠血行血常规、ALT、cr分析。结果：各组间裸鼠体重差异无统计学意义（P〉0．05）。裸鼠接种成瘤率100％，各组瘤体积：组4〈组5〈组1〈组2〈组3〈PG〈NG，差异均有统计学意义（P〈0．05）。各组移植瘤MVD：组4〈组5〈组1〈组2〈组3〈PG〈NG，差异有统计学意义（P〈0．05）。各组移植瘤NF—κB表达：组4〈组5〈组3〈PG〈组1〈组2〈NG，差异有统计学意义（P〈0．05）。实验组肺、肝、肾组织细胞结构及裸鼠血常规、ALT、Cr检测结果与阴性对照组比较，差异无统计学意义（P〉0．05）。结论：Tha联合AsT能够降低移植瘤MVD，下调NF—κB表达，具有协同抑瘤作用，毒副反应轻微，值得进一步研究。

The joint effects and toxicities of Thalidomide and Arsenic Trioxide in the prevention of SMMC7721 xenograft tumor growth in nude mice

Objective：To observe the therapeutical and side effects of thalidomide （Tha）, arsenic trioxide （AsT）, Tha joint AsT on the growth of SMMC7721 xenografts, the tumor microvessel density （MVD） and nuclear factor kappa B （NF- KB） were also detected. Methods：SMMC7721 cells were subcutaneously injected on 35 nude mice which were randomly divided into seven groups ： high dose Tha （ HTha, group 1 ）, low dose Tha （ LTha, group 2 ）, AsT （ group 3 ）, HTha ＋ AsT （ group 4 ）, LTha ＋ AsT （ group 5 ）, negative group （NG） and positive group （ PG ）, each group had 5 nude mice. A week later, drugs were administrated in group 1, group 2 and the NG every Monday, Wednesday and Friday, group 3 and the PG were injected every Monday, group 4 and group 5 were treated as former, each group had been administrated for four weeks. The weight of nude mice and the volume of the xenografts were mo- nitored, the action and reactions of nude mice were also observed. Four weeks later, the mice were sacrificed, the tumor MVD and the expression of NF - KB were detected, the xenografts,lungs,livers, kidneys were taken for pathologic ex- amination, the blood routine, alanine aminotransferase （ ALT）, ereatinine （Cr） were analyzed. Results： There was no significant difference on the weight of nude mice in each group （ P 〉 0.05 ）. SMMC7721 cells were successfully inocu- lated on each nude mouse. The results of tumor volume as following： group 4 〈 group 5 〈 group 1 〈 group 2 〈 group 3 〈 PG 〈 NG （ P 〈 0.05 ） ; The tumor MVD of each group ： group 4 〈 group 5 〈 group 1 〈 group 2 〈 group 3 〈 PG 〈 NG, the difference was statistically significant （ P 〈 0.05 ）. Expression of NF - KB ： group 4 〈 group 5 〈 group 3 〈 PG 〈 group 1 〈 group 2 〈 NG, the difference was statistically significant （ P 〈 0.05 ）. As to the changes of the cell structure about lung ,liver,kidney and the blood routine,ALT,Cr,there were no significant differences in