5/6肾切除大鼠肾脏病理图像分析与中医证型的研究

目的 :探讨 5 / 6肾切除 (5 / 6nephrectomy ,5 / 6NT)大鼠肾脏病理动态变化与图像分析、中医证型的关系。方法 :5 / 6NT大鼠肾组织标本的PAS、PAM染色片 ,用图像分析仪分别对PAM染色片的肾小球直径、肾小球截面积、系膜基质及血管壁面积进行定量分析 ,观察肾脏病理动态变化情况。结果 :5 / 6NT大鼠肾脏病理变化的基本特征为肾小球系膜细胞 (glomenrulusmesangialcells,GMC)增生 ,肾小管扩张 ,肾间质炎症反应 ,肾小管蛋白管型形成 ,并逐渐形成肾小球纤维化、硬化。这些病理变化与中医“瘀血”、“瘀浊”的概念相符合。病理变化轻重与肾小球直径、系膜基质面积、肾小球截面积、肾小球体积及血管壁面积肾小球基底膜、肾小球及肾血管壁面积呈正比例关系。结论 :肾小球直径、系膜基质面积、肾小球截面积、肾小球体积及血管壁面积肾小球基底膜、肾小球及肾血管壁面积可以作为肾纤维化、硬化的评价指标 ;5 / 6NT大鼠肾脏病理相当于中医的“瘀血”、“瘀浊”证

The Image Analysis and Traditional Chinese Medicine Syndrome and Renal Pathology Dynamic Change of 5/6 Nephrectomy

Objective:To study the relation of the image analysis and Gaditional Chinese Medicine syndrome and renal pathology dynamic change of 5/6 nephrectomy rats.Methods:Observed the renal pathology dynamic change of periodic acidsilver metheramine(PAM) of renal tissue by image analysis and periodic acid shiff(PAS).Results:The results showed that:characteristic of renal pathology dynamic change of 5/6 nephrectomy:glomenrulus mesangial cells hyperplasia,tubule expansion ,tubule-interstitial inflammation,protein tube of tubule,developing glomenrulus fibrosis and glomenrulosclerosis.It's equivalent to Traditional Chinese Medicine blood stasis and blood turbid.In renal pathology change and glomenrulus diameter ,glomenrulus mesangial area,glomenrulus area,glomenrulus cubage,renal blood vessel wall area are direct proportion .Conclusion:The glomenrulus diameter,glomenrulus mesangial area,glomenrulus area,glomenrulus cubage,renal blood vessel wall area can be used to assess target fibrosis and glomenrulosclerosis.5/6 nephrectomy pathology is equivalent to Traditional Chinese Medicine blood stasis and blood turbid.