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| 脑内注射脂多糖后常驻小胶质细胞死亡而中性粒细胞和单核细胞浸润成为主要炎症细胞 | |
| 作者姓名： | 王雪贞 |
| 作者单位： | Neuroscience Graduate Program Ajou University School of Medicine，Suwon，Korea Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea Brain Disease Research Center，Ajou University School of Medicine，Suwon，Korea，Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea，Neuroscience Graduate Program，Ajou University School of Medicine，Suwon，Korea Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea Brain Disease Research Center，Ajou University School of Medicine，Suwon，Korea，Neuroscience Graduate Program，Ajou University School of Medicine，Suwon，Korea Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea Brain Disease Research Center，Ajou University School of Medicine，Suwon，Korea，Department of Radiation Oncology，Ajou University School of Medicine，Suwon，Korea，Neuroscience Graduate Program，Ajou University School of Medicine，Suwon，Korea Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea Chronic Inflammatory Disease Research Center，Ajou University School of Medicine，Suwon，Korea，Neuroscience Graduate Program，Ajou University School of Medicine，Suwon，Korea Department of Pharmacology，Ajou University School of Medicine，Suwon，Korea Brain Disease Research Center，Ajou University School of Medicine，Suwon，Korea |
| 摘 要： | 为了研究小胶质细胞和血中炎症细胞对脑炎症的影响,在正常和白细胞减少大鼠的黑质致密部(SNpc)、皮层和海马注射脂多糖(LPS),用特异标记来研究中性粒细胞、小胶质细胞和单核细胞的行为学。CD11b+和Iba-1+阳性细胞在正常脑和LPS注射后6h反应相似。注射后12hIba-1+细胞消失而CD11b+变成圆形。CD11b/Iba-1双阳性网状小胶质细胞在LPS注射后6h死亡。注射后12h检测到的CD11b+细胞是MPO+细胞。这些CD11b+/MPO+细胞在白细胞减少的大鼠脑内不出现,提示中性粒细胞浸润。MPO+中性粒细胞表达一氧化氮合酶(iNOS)、IL-1β、环氧合酶-2(COX-2)和单核细胞趋化蛋白-1(MCP-1),但在注射后18h死亡。在24h检测到的CD11b+细胞是浸润的单核细胞,因为这些细胞曾经是Iba-1+而且在白细胞减少的大鼠脑内不出现。而且,在LPS注射的脑内可以检测到移植的单核细胞。这些结果提示,在发生炎症的脑内,至少有一部分中性粒细胞和单核细胞被认为是活化的小胶质细胞。 |
| 关 键 词： | 小胶质细胞 单核细胞 中性粒细胞 神经炎症 |
| 文章编号： | 1001-117X(2008)01-0047-06 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |