尿道炎后慢性前列腺炎患者精浆中TNF-α及IL-18的检测及临床意义

目的检测尿道炎后慢性前列腺炎患者精浆中肿瘤坏死因子(TNF-α)、白细胞介素18(IL-18)的浓度并探讨其临床意义。方法采用ELISA法测定精浆中IL-18浓度;放射免疫法测定精浆中TNF-α浓度。结果尿道炎后慢性前列腺炎(Ⅱ型、Ⅲ型)患者精浆中TNF-α浓度与对照组比较,差异均有显著性(P<0.01);而在慢性细菌性前列腺炎(Ⅱ型)和慢性非细菌性前列腺炎(Ⅲ型)之间比较,差异无显著性(P>0.05)。尿道炎后慢性前列腺炎(Ⅱ型、Ⅲ型)患者精浆中IL-18浓度与对照组、Ⅱ型和Ⅲ型比较,差异均有显著性(P<0.01)。结论尿道炎后慢性前列腺炎患者精浆中TNF-α、IL-18明显升高,提示TNF-α,IL-18在尿道炎后慢性前列腺炎的发病过程中起了重要的作用。

Clinical Evaluation of IL-18 and TNF-α in Seminal Plasma of Chronic Prostatitis after Genital Tract Infections

Objective To determine the clinical significance of interleukin-18(IL-18)and tumor necrosis factor-α(TNF-α)in expressed seminal plasma(SP)for chronic prostatitis(CP)after genital tract infections.Methods IL-18 in SP were examined by enzyme-linked immunosorbent assay(ELISA);TNF-α in SP were examined by radioimmunosassay.Results Mean levels of TNF-α were higher in SP from CP(Ⅱ,Ⅲ type)after genital tract infections compared with SP from normal controls(P<0.01);however,no significant differences could be detected between Ⅱtype and Ⅲ type(P>0.05).Not only there were statistically significant between levels of IL-18 in SP from CP(Ⅱ,Ⅲ type)after genital tract infections and SP from normal controls(P<0.01);but also there were statistically significant of levels of IL-18 in SP between Ⅱtype and Ⅲ type(P<0.01).Conclusion The levels of IL-l8 and TNF-α in SP of CP after genital tract infections expressed very high.Cytokines may play an important role in CP after genital tract infections.The result indicates that the expression of cytokines(TNF-α,IL-18)in SP can serve as a valuable marker for the diagnosis of CP.