2003-2005年河北省急性弛缓性麻痹实验室监测结果分析

目的 及时发现可能出现的脊灰疫苗衍生株(VDPV)或输入脊灰野病毒,以防止其扩散.方法 按世界卫生组织<脊髓灰质炎病毒检验手册>对河北省2003-2005年急性弛缓性麻痹病例粪便标本进行病毒分离与血清定型,所有标本用L20B、RD和Hep-2等3种细胞同时进行脊灰病毒(PV)和肠道病毒分离,用PCR-RFLP法和ELISA法做型内鉴定.结果 2003-2005年共采集了1 199例急性弛缓性麻痹(AFP)病例粪便标本.分离到脊灰病毒(PV)86株,非脊灰肠道病毒(NPEV)115株.从AFP病例及其高危病例的5份密切接触者粪便标本中共分离到PV 100株,用血清型中和试验和PCR-RFLP法进行型内鉴定,结果为Ⅰ型12株,Ⅱ型29株,Ⅲ型23株,混合型23株,PV NPEV 8株,疫苗变异株5株.将PV混合株进行单型分离后,所有单型毒株共138株,经ELISA试验鉴定,其中疫苗类似株(SL)132株(95.7%),双反应株(DRV)5株(3.8%),非疫苗类似株(NSL)1株(0.7%).结论 河北省2003-2005年未发现疫苗衍生脊灰病毒或输入性脊灰野病毒,继续保持无脊灰状态.

Laboratory Surveillance of Acute Flaccid Paralysis in Hebei Province

Objective To study the surveillance indicators in polio laboratory of Hebei,so as to surveillance VDPV or wild-type poliovirus in time.Methods According to WHO's normal method to isolate virus by L20B,RD and Hep-2 cells,and then the neutralization test,intratypic differentiation test were carried out to all poliovirus isolated using PCR-RFLP and ELISA methods.Results All the surveillance indicators in polio laboratory of Hebei met the requirements of WHO and MOH.There were 1 199 stool samples collected in 2003-2005. 86 polioviruses were isolated from AFP cases,and 115 of non-polio enteroviruses(NPEV) were also isolated from AFP cases.Among 100 positive isolates,12 of type 1 vaccine-like viruses,29 of type 2 vaccine-like viruses,23 of type 3 vaccine-like viruses,23 of mixed viruses,8 of vaccine-like viruses mixed with enteroviruses,and 5 of mutant viruses were identified by PCR-RFLP method.Then ELISA test was conducted according to WHO manual,and among total 138 single serotype viruses,132(95.7%) of sabin-like viruses(SL),5(3.8%) of double reaction viruses(DRV),1(0.7%) of non-sobilike viruses(NSL) were identified.Conclusion No wild polioviruses or VDPVs were found in Hebei.maintaining a nopolio wild-type virus status in Hebei.