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Laboratory procedures for the detection and identification of cutaneous non‐tuberculous mycobacterial infections
Authors:Kazue NAKANAGA  Yoshihiko HOSHINO  Rie R. YOTSU  Masahiko MAKINO  Norihisa ISHII
Affiliation:1. Leprosy Research Center, National Institute of Infectious Diseases, , Tokyo, Japan;2. Department of Dermatology, National Center for Global Health and Medicine, , Tokyo, Japan
Abstract:There is evidence that the incidence of cutaneous non‐tuberculous mycobacterial (NTM) infection is increasing worldwide. Novel culture methods and new analytical procedures have led to significant advancements in understanding the origin and progression of NTM infections. Differential identification of NTM isolates is important because culture characteristics and/or sensitivity to anti‐mycobacterium drugs vary between different mycobacterial species. In this manuscript, we describe the latest diagnostic techniques for cutaneous NTM infection and show how these methodologies can be used for the diagnosis of Buruli ulcer in Japan.
Keywords:16S rRNA gene  Buruli ulcer  cutaneous infection  non‐tuberculous mycobacterial infection  polymerase chain reaction  species identification
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