Rosmarinic acid protects on rat bone marrow mesenchymal stem cells from hydrogen peroxide-induced apoptosis |
| |
| Authors: | Li-Zhen Lin Huan-Huan Chen Zhou-Xi Lei Yun-Rong Li Chun-Hua Zhou Yue-Chun Huang |
| |
| Institution: | 1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;2. The First affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, China |
| |
| Abstract: | To investigate the anti-oxidant activities and mechanism of rosmarinic acid (RA) on rat bone marrow mesenchymal stem cells (rBMSCs) from ischemia-induced apoptosis in vitro, which was established using H2O2-damage and analyzed for cell viability, cell apoptosis, ROS, morphological changes, and levels of apoptosis proteins. Pretreatment with RA significantly suppressed the generation of ROS, protected the morphological changes of cells, decrease the ratio of cell apoptosis, down-regulated the level of caspase-3, caspase-9, Bax/Bcl-2, and up-regulated the level of p-PI3K. These findings suggest that RA may protect rBMSCs from H2O2-induced apoptosis by partly regulating PI3K/Akt signaling pathway and can be developed as a potential anti-apoptotic agent for therapy in cardiovascular diseases. |
| |
| Keywords: | Rosmarinic acid rBMSCs oxidative stress ROS apoptosis PI3K/Akt signaling pathway |
|
|
