| 钙通道阻滞剂维拉帕米抑制肝星状细胞活化和功能 |
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| 引用本文： | 戴夫,李海文,彭琼,李元元,林沪.钙通道阻滞剂维拉帕米抑制肝星状细胞活化和功能[J].胃肠病学和肝病学杂志,2011,20(4):323-326. |
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| 作者姓名： | 戴夫 李海文 彭琼 李元元 林沪 |
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| 作者单位： | 1. 安徽医科大学第三附属医院消化内科,安徽,合肥230061
2. 解放军302医院生物治疗研究中心 |
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| 摘 要： | 探讨维拉帕米（Ver）对人肝星状细胞系（HSC）-LX2的活化及分泌转化生长因子（TGF-β）的抑制作用。方法体外用内皮素-1（endothelin,ET-1）刺激HSC-LX2活化建立培养体系,设对照、ET-1和ET-1＋Ver 3组,对照组仅加入培养基,ET-1组加入ET-1和培养基,ET-1＋Ver组加入ET-1、Ver和培养基,分别在（0、12、24、48、72 h）观察HSC分沁α-平滑肌肌动蛋白（α-SMA）和细胞因子的能力,采用细胞爬片和免疫组化技术鉴定活化HSC的细胞形态;流式细胞术分析HSC表达α-SMA水平,ELISA测定不同组HSC分泌TGF-β的浓度。结果与对照组比较,ET-1组HSC活化和分泌TGF-β的能力较强（P〈0.05）;与ET-1组比较,ET-1＋Ver组HSC活化和分泌TGF-β能力较弱（P〈0.05）。结论 Ver体外实验中能抑制HSC的增殖和活化,具有潜在的抗纤维化作用。
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| 关 键 词： | 维拉帕米 肝硬化 星状细胞 α-平滑肌纤维 转化生长因子β |
Verapamil inhibits the activation and function of hepatic stellate cells |
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| DAI Fu,LI Haiwen,PENG Qiong,LI Yuanyuan,LIN Hu.Verapamil inhibits the activation and function of hepatic stellate cells[J].Chinese Journal of Gastroenterology and Hepatology,2011,20(4):323-326. |
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| Authors: | DAI Fu LI Haiwen PENG Qiong LI Yuanyuan LIN Hu |
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| Institution: | DAI Fu1,LI Haiwen1,PENG Qiong1,LI Yuanyuan2,LIN Hu2 1.Department of Gastroenterology,the Third Affiliated Hospital of Anhui Medical University,Hefei 230061,2.Research Center for Biological Therapy,302 Hospital of PLA,China |
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| Abstract: | Objective To investigate the effect of Verapamil on the regulation of proliferation and function of human hepatic stellate cell lines(HSC),and illuminate its further pharmacological role.Methods Activation of HSC culture system stimulated by ET-1 was established in vitro,which was divided into three groups: control group,ET-1 group and ET-1+Verapamil group.The control group was added with medium only.The ET-1 group was added with medium and ET-1.The ET-1+Verapamil group was added with medium,ET-1 and Verapa... |
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| Keywords: | Verapamil Liver cirrhosis Hepatic stellate cells α-SMA TGF-β |
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