| 反义生存素诱导胃癌细胞凋亡的实验研究 |
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| 引用本文： | 陈曦海,张岩,纪艳超,翟哲,吴波.反义生存素诱导胃癌细胞凋亡的实验研究[J].胃肠病学和肝病学杂志,2009,18(11). |
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| 作者姓名： | 陈曦海 张岩 纪艳超 翟哲 吴波 |
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| 作者单位： | 哈尔滨医科大学附属第四医院普外科,黑龙江,哈尔滨,150001 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 目的构建反义生存素真核表达载体(anti-pcDNA3-svv),探讨其对胃癌的凋亡诱导作用。方法采用反义生存素核酸瞬时电转染法转染胃癌细胞系Skov3,筛选出阳性细胞株。以Western blot检测生存素蛋白的表达,用流式细胞仪检测其对Skov3的凋亡诱导作用,并检测实验组细胞(Skov3-SVVanti)MDR-1 mRNA的表达。结果稳定表达anti-pcDNA3-svv的Skov3-SVVanti组生存素蛋白表达较Skov3-neo和Skov3组明显降低,Skov3-SVVanti组细胞流式细胞仪检测显示其凋亡率为19%。Skov3-SVVanti组MDR-1 mRNA表达较Skov3和Skov3-neo组明显减少(P<0.01)。结论反义生存素核酸可诱导胃癌细胞凋亡,并能降低MDR-1mRNA表达。
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| 关 键 词： | 反义生存素核酸 胃癌 凋亡 |
Experimental study of gastric cancer cell apoptosis induced by survivin antisense RNA |
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| CHEN Xihai,ZHANG Yan,JI Yanchao,ZHAI Zhe,WU Bo.Experimental study of gastric cancer cell apoptosis induced by survivin antisense RNA[J].Chinese Journal of Gastroenterology and Hepatology,2009,18(11). |
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| Authors: | CHEN Xihai ZHANG Yan JI Yanchao ZHAI Zhe WU Bo |
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| Institution: | CHEN Xihai; ZHANG Yan; JI Yanchao; ZHAI Zhe; WU Bo Department of General Surgery; the Fourth Hospital of Harbin Medical University; Harbin 150001; China; |
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| Abstract: | Objective To establish survivin antisense RNA and to explore its effect on the apoptosis of gastric cancer cells.Methods Survivin antisense eukaryotic vector anti-pcDNA3-svv was transferred into Skov3 cells by electroporation and positive clone was screened out.Survivin protein was detected by Western blot;the effect of apoptosis inducement was observed by flow cytometry;the expression of MDR-1 mRNA was detected.Results Survivin protein of positive clone cells reduced obviously compared with Skov3-neo and S... |
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| Keywords: | Survivin antisense RNA Gastric cancer Apoptosis |
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