| 婴儿双歧杆菌介导的CD／UPRT双自杀基因系统对黑色素瘤细胞的杀伤作用 |
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| 引用本文： | 郭志英,任启伟,安丽娜.婴儿双歧杆菌介导的CD／UPRT双自杀基因系统对黑色素瘤细胞的杀伤作用[J].济宁医学院学报,2009,32(1):24-26. |
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| 作者姓名： | 郭志英 任启伟 安丽娜 |
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| 作者单位： | 1. 济宁医学院基础学院
2. 四川大学华西医学中心 |
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| 摘 要： | 目的探讨尿嘧啶磷酸核糖转移酶基因（UPRT）对胞嘧啶脱氨酶／5-氟胞嘧啶（CD／5-FC）自杀基因系统抗瘤作用的增强效应。方法构建重组质粒pGEX-UPRT，以电穿孔法转化婴儿双歧杆菌，筛选出阳性克隆菌，加入5-FC厌氧条件下培养24h后取其上清，加到B16-F10细胞培养液中，采用MTT法检测细胞生存率，并观察细胞形态学改变。结果阳性克隆菌可正确表达UPRT基因。与对照组比较，CD组、UPRT组及CD／UPRT组细胞存活率均明显下降；与CD组及UPRT组比较，CD／UPRT组细胞存活率明显降低。且CD／UPRT组肿瘤细胞显示出明显的损伤性改变，而CD组、UPRT组变化不如前者。结论婴儿双歧杆菌介导的UPRT基因可明显增强CD／5-FC系统对鼠黑色素瘤细胞B16-F10的杀伤作用。
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| 关 键 词： | 婴儿双歧杆菌 尿嘧啶磷酸核糖转移酶 胞嘧啶脱氨酶 肿瘤 治疗 |
The Killing Effect of Bifidobacterium Infants-medicated CD/UPRT Double Suicide Gene on Melanoma |
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| GUO Zhiying,REN Qiwei,AN Lina.The Killing Effect of Bifidobacterium Infants-medicated CD/UPRT Double Suicide Gene on Melanoma[J].Jounal of Jinning Medical College,2009,32(1):24-26. |
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| Authors: | GUO Zhiying REN Qiwei AN Lina |
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| Institution: | Jining Medical College |
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| Abstract: | Objective To evaluate the UPRT-enhanced antitumor effect of CD/5-FC suicide gene system.Methods Recombined plasmid pGEX-UPRT was constructed and transferred into bifidobacterium infants by electroporation.Positive recombined bacteria was selected and identified,and then was incubated with 5-FC in anaerobic condition.The supernatant of bacteria was collected and added to the melanoma B16-F10 cells.The cell growth status was evaluated by MTT assay,and the morphologic changes of cells were observed.Results Rec... |
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| Keywords: | bifidobacterium infants uracil phosphoribosyltransferase （UPRT） cytosine deaminase （CD） tumor therapy |
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