An ELISA method for quantification of Tamm-Horsfall protein using monoclonal antibodies |
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| Authors: | B Kjellsson T S?derstr?m L A Hanson |
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| Affiliation: | 1. Department of Pediatric Surgery, Sami Ulus Women''s Health and Children Training and Research Hospital, Ankara, Turkey;2. Department of Urology, Oncology Training and Research Hospital, Ankara, Turkey;3. Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey;4. Department of Pathology, Sami Ulus Women''s Health and Children Training and Research Hospital, Ankara, Turkey |
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| Abstract: | ![]()
Eight hybridoma cell lines secreting monoclonal antibodies (MoAbs) to Tamm-Horsfall protein (THP) were established. The isotype and reaction pattern of the MoAbs with THP from rat, rabbit, guinea pig and man were employed for the selection of clones. At least four epitopes were recognised on human THP. One of these epitopes differed from the others in its dependence on the state of aggregation of the THP. An ELISA procedure was developed for quantification of THP in urine requiring no other sample treatment than dilution in the assay buffer. In this ELISA, THP showed an increased immunoreactivity after freezing. |
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