AZT对SGC7901胃癌细胞凋亡及Bax蛋白和PCNA表达的影响

目的:研究端粒酶抑制剂3′-叠氮3′-脱氧胸腺核苷(AZT)对SGC 7901胃癌细胞凋亡及Bax蛋白和增殖细胞核抗原(PCNA)表达的影响,探讨其诱导肿瘤细胞凋亡的可能机制.方法:将处于对数生长期的SGC 7901胃癌细胞分为对照组及1.0 mmol·L-1 AZT处理组,应用AZT 6 d后,采用MG-P-MY组合...

Influence of AZT on apoptosis of gastric cancer cell line SCG 7901 and expressions of Bax protein and PCNA

Objective To study the influence of 3′-azido-3′-deoxythymidine(AZT) on the apoptosis of gastric cancer cell line SCG 7901 and the expressions of BAX protein and proliferation cell nuclear antigen(PCNA) and explore the mechanisms.Methods The gastric cancer cell line SCG 7901 treated with 1.0 mmol·L-1 AZT for 6 d were used as AZT group,at the same time,control group was set up.The apoptotic rates of SGC 7901 in two groups were detected with MG-P-MY staining.The expressions of Bax protein and PCNA of gastric cancer cell line SCG 7901 6 d after drug administration were determined by immunohistochemical technique(EnVision).Results The apoptotic rates in AZT proup and control group were(16.421±0.713)% and(3.366±0.271)% respectively,the apoptotic rate in AZT group was significantly higher than that in control group(P<0.05).Compared with control group,the expression of Bax protein was significantly increased(16.851%±10.064% vs 47.389%±18.042%,P<0.05) and the expression of PCNA was significantly decreased(23.446%±13.055% vs 62.332±22.767%,P<0.05) in AZT group.Conclusion AZT can induce the apoptosis of gastric cancer cell line SCG 7901,promote the expression of Bax protein and inhibit the expression of PCNA.