Notch配体Delta-1ike1对小鼠骨髓细胞来源的树突状细胞分化和抗原呈递功能的影响

本研究探讨Notch配体Delta—likel（Dil1）对小鼠骨髓细胞来源的树突状细胞（dendriticcell，DC）分化及其抗原呈递功能的影响。在GM—CSF和IL4存在条件下，用OP9-Dil1和OPt）．GFP细胞分别与小鼠骨髓细胞共培养8天，经肿瘤抗原刺激成熟。用流式细胞仪检测DC表面MHCII、CD80和CD86的表达情况，ELISA法检测肿瘤抗原刺激后DC培养上清细胞因子IL-12和IL110的水平，通过混合T淋巴细胞反应观察DC对T细胞的促增殖能力。结果表明：与GFP组相比，Dll1组的小鼠骨髓来源的树突状细胞明显增多（P〈0．05）。肿瘤抗原刺激后，Dll1组DC表面MHCII、CD80和CD86表达量更高。DC分泌的IL-12水平显著高于对照组（P〈0．05），而IL-10的水平显著低于对照组（P〈0．01）。Dll1组DC具有更强的促T细胞增殖活性。结论：OP9-Dll1促进小鼠骨髓细胞向DC分化并增强其抗原呈递功能。

Role of Delta-like 1 in Differentiation and Antigen Presentation of Mouse Bone Marrow-Derived Dendritic Cells

The aim of this study was to investigate the role of Delta-like 1 （ Dill ） in diffrentiation and antigen presentation of mouse bone marrow-derived dendritic cells （DCs）. In the presence of granulocyte macrophage colony stimulating factor （GM-CSF） and interleukin 4 （1L-4）, mouse bone marrow cells were co-cultured with OP9-Dlll and OP9- GFP cell lines respectively. After 8 days, the immature DCs were stimulated with tumor antigen. The surface molecules of the activated DCs including MHC II, CD80 and CD86 were analyzed by flow cytometry. Levels of IL-12 and IL-10 in the culture supernatant were detected by ELISA. In addition, the proliferation of T-cells co-cultured with DCs was analyzed by FACS through mixed T-lymphocyte reaction. The results showed that compared with OP9-GFP, the bone marrow cells co-cultured with OP9-Dlll produced significantly more CD11 c ^＋ DCs （p 〈 0.05 ）, and possessed higher levels of surface molecule expression including MHC II, CD80 and CD86 after tumor antigen stimulation. The DCs secreted higher level of IL-12（p 〈0.05） and less IL-10 （p 〈0.01）. They also resulted in significantly stronger T-cell proliferation response. It is concluded that Dill can promote the differentiation of DCs from mouse bone marrow ceils and enhance their antigen presentation capacity.