| PD 98059对食管癌细胞放射敏感性影响的研究 |
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| 引用本文： | 吴珍,李胜保,童强. PD 98059对食管癌细胞放射敏感性影响的研究[J]. 临床消化病杂志, 2009, 21(4): 198-200 |
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| 作者姓名： | 吴珍 李胜保 童强 |
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| 作者单位： | 郧阳医学院附属太和医院消化内科,十堰,442000;郧阳医学院附属太和医院消化内科,十堰,442000;郧阳医学院附属太和医院消化内科,十堰,442000 |
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| 摘 要： | 目的 探讨PD 98059通过抑制丝裂原活化蛋白激酶-细胞外信号调节激酶(MRPK-ERK)信号通路是否改变食管癌细胞的放射敏感性.方法 用食管癌细胞株EC 9706为实验对象,分别接受单纯放射、PD 98059和二者结合处理.通过Western印记法证实PD 98059可下调ERK活性.采用成克隆法定量分析细胞增殖性死亡.通过流式细胞仪检测细胞凋亡情况.结果 单纯PD 98059(10μmoL/L)可抑制ERK的磷酸化,而单纯放射对ERK的活性无明显影响,二者结合可提高对ERK活性的抑制作用.PD 98059(10 μmol/L)在放射前与细胞作用1 h及放射后作用10 d均可提高EC 9706细胞对放射的敏感性.PD 98059结合放射可增加EC 9706细胞增殖性死亡,D0值的放射增敏比为1.69.PD 98059可增加EC 9706细胞放射后诱导的细胞凋亡.结论 PD 98059通过抑制MAPK降低ERK活性,增加EC 9706细胞对放射的敏感性,为筛选放射敏感剂临床实验提供了依据.
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| 关 键 词： | 食管癌 PD98059 丝裂原活化蛋白激酶-细胞外信号调节激酶 放射敏感性 |
PD 98059 Influence the Radiosensitivity of Esophageal Carcinoma Cells |
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| WU ZHEN,LI Sheng-bao,TONG Qiang. PD 98059 Influence the Radiosensitivity of Esophageal Carcinoma Cells[J]. Chinese Journal of Clinical Gastroenterology, 2009, 21(4): 198-200 |
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| Authors: | WU ZHEN LI Sheng-bao TONG Qiang |
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| Affiliation: | WU ZHEN,LI Sheng-bao,TONG Qiang Deparement of Gastroenternology,Taihe Hospital Affiliated to Yunyang Medical College,Shiyan,442000,China |
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| Abstract: | Objective To evaluate whether PD 98059,suppressing MAPK-ERK signaling pathway,can change the sensitivity of esophageal carcinoma cells to radiotherapy.Methods Esophageal carcinoma cells EC 9706 treated with:radiation alone;PD 98059;or the combination of radiation and PD 98059.The inhibition of MAPK-ERK by PD 98059 was confirmed by Western blot.Clonogenic assay was used quantitatively to measure the mitotic cell death and flow cytometry was used to evaluate apoptosis.Results(1)PD 98059 could partially inhibt... |
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| Keywords: | PD98059 |
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